One "NIH" unit of thrombin is equivalent to 1.1 to 1.3 International Units, depending on the influence of polyethylene glycol in the assay (Thromb Haemost. 1995 Sep;74(3):900-3). A unitage ratio of 1.15 is recommended based on data obtained in the presence and absence of polyethylene glycol in comparative assays.
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一般說明
凝血酶是止血过程中的最终凝血蛋白酶，可同时促进促凝血和抗凝血作用。
應用
凝血酶被用于重组融合蛋白的位点特异性切割，这些重组融合蛋白具有可接近的凝血酶识别位点以除去亲和标签。已有研究使用凝血酶比较了低分子量肝素（LMWH）与其他抗凝剂作为大型矫形手术的静脉血栓栓塞预防剂的疗效和安全性。 [1]
生化／生理作用
选择性裂解纤维蛋白原中 Arg-Gly 键形成纤维蛋白和纤维蛋白肽 A 和 B 的丝氨酸蛋白酶。
單位定義
活性以 NIH 单位表示，通过与 NIH 凝血酶参考标准品的直接比较获得
重構
当使用1 ml H2O重悬时，一瓶含有10 mg牛血清白蛋白、0.15 M NaCl和0.05 M柠檬酸钠，pH 6.5
分析報告
NIH 试验步骤基于 Biggs 方法的改进，使用 0.2mL 稀释血浆（与生理盐水 1:1 混合，作为底物）和 0.1mL Biggs 凝血酶样品（稳定于 1% 缓冲白蛋白溶液中）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。
其他說明
查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。
免責聲明
供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
基底
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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What is the equivalence in UI of 1 NIH of trombine
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