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Merck
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T9326

Sigma-Aldrich

凝血酶 人

BioUltra, recombinant, expressed in HEK 293 cells, aqueous solution, ≥95% (SDS-PAGE)

同義詞:

IIa 因子

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About This Item

酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

重組細胞

expressed in HEK 293 cells

品質等級

產品線

BioUltra

化驗

≥95% (SDS-PAGE)

形狀

aqueous solution

比活性

≥2,000 units/mg protein

分子量

37.4 kDa

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... F2(2147)

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一般說明

凝血酶是止血方面的最终凝血蛋白酶,促进促凝血和抗凝血作用。

應用

凝血酶被用于重组融合蛋白的位点特异性切割,这些重组融合蛋白具有可接近的凝血酶识别位点以除去亲和标签。凝血酶已用在一项评估法国冻干血浆的体外止血特性的研究中。

單位定義

活性以NIH单位表示,它是通过与NIH凝血酶参考标准品直接对比而获得的。

外觀

以 20mM MES,pH 6.0,500mM 氯化胆碱的溶液形式提供

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Katerina Zakharova et al.
International journal of molecular sciences, 22(14) (2021-07-25)
Redβ is a 261 amino acid protein from bacteriophage λ that promotes a single-strand annealing (SSA) reaction for repair of double-stranded DNA (dsDNA) breaks. While there is currently no high-resolution structure available for Redβ, models of its DNA binding domain
Ute Metzger et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(34), 14309-14314 (2009-08-27)
Ergot alkaloids are toxins and important pharmaceuticals that are produced biotechnologically on an industrial scale. The first committed step of ergot alkaloid biosynthesis is catalyzed by dimethylallyl tryptophan synthase (DMATS; EC 2.5.1.34). Orthologs of DMATS are found in many fungal
Christophe Martinaud et al.
Anesthesiology, 117(2), 339-346 (2012-06-29)
French lyophilized plasma (FLyP) is used routinely by the French Armed Forces in war settings. The authors compared concentrations of coagulation proteins and global in vitro hemostatic properties in FLyP and in the same plasma before lyophilization to assess the
Dmitriy Krepkiy et al.
Nature, 462(7272), 473-479 (2009-11-27)
Despite the growing number of atomic-resolution membrane protein structures, direct structural information about proteins in their native membrane environment is scarce. This problem is particularly relevant in the case of the highly charged S1-S4 voltage-sensing domains responsible for nerve impulses
Diane Moujalled et al.
Cell death & disease, 12(1), 133-133 (2021-01-30)
Necroptosis is a pro-inflammatory cell death program executed by the terminal effector, mixed lineage kinase domain-like (MLKL). Previous studies suggested a role for the necroptotic machinery in platelets, where loss of MLKL or its upstream regulator, RIPK3 kinase, impacted thrombosis

文章

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

凝血酶原因子 IIa 是一種內溶性絲氨酸蛋白酶,可選擇性地裂解纖維蛋白原的 Arg-Gly 鍵,形成纖維蛋白,並釋放出纖維蛋白肽 A 和 B。

Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.

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