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T0565

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

TMB membrane substrate, chromogenic, liquid

同義詞:

TMB membrane substrate

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83
暫時無法取得訂價和供貨情況

產品名稱

用于膜的3,3′,5,5′-四甲基联苯胺（TMB）液体底物系统, ready to use solution

品質等級

100

形狀

liquid

儲存溫度

2-8°C

相關類別

一般說明

3,3′,5,5′-四甲基联苯胺(TMB)是辣根过氧化物酶(HRP)偶联物的显色底物。它会产生永久性不可溶解的深蓝色反应产物，可应用于比色定量。[1]

應用

3,3′,5,5′-四甲基联苯胺(TMB)显色液（转印膜用底物）可作为蛋白质免疫印迹分析(western blot)的显色底物。也可用作酶联免疫吸附测定(ELISA)的底物溶液。
3,3′,5,5′-四甲基联苯胺(TMB)细胞膜的液体底物体系：
  • 用于酶联免疫斑点法(ELISPOT)分析[2]
  • 用作IgG过氧化物酶的底物[3]
  • 应用于免疫复合物的可视化[4]

外觀

即用型。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

Lot/Batch Number
0000391229
0000391229
0000358193
0000392439
0000376027

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Navilla Apú et al.
Open life sciences, 18(1), 20220577-20220577 (2023-08-17)
Most laboratory tests to detect the presence of anti-SARS-CoV-2 antibodies use enzyme-linked immunosorbent assays (ELISA) or chemiluminescence immunoassays (CLIA); however, equipment for these immunoassays is unavailable in many areas of low- and middle-income countries. Rapid lateral flow immunoassay (LFIA) tests
Effects of nerve growth factor antagonist K252a on peritoneal mast cell degranulation: implications for rat postoperative ileus
Berdun S, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 309(10), G801-G806 (2015)
Muhammed M Salahuddin et al.
Current issues in molecular biology, 43(3), 2199-2209 (2021-12-24)
Breast cancer is the most common malignancy in women worldwide. P2X7 is a transmembrane receptor expressed in breast cancer and activated by the ATP tumor microenvironment, driving cell proliferation, angiogenesis, and metastasis via different signaling pathways. The role of the
immune response of multiparous hyper-immunized sows against peptides from non-structural and structural proteins of PRRSV
Rascon-Castelo E, et al.
Vaccines, 3(4), 973-987 (2015)
Srdjan M Dragovic et al.
Cell host & microbe, 23(4), 523-535 (2018-04-13)
Plasmodium infection begins with the bite of an anopheline mosquito, when sporozoites along with saliva are injected into a vertebrate host. The role of the host responses to mosquito saliva components in malaria remains unclear. We observed that antisera against
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文章

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

使用多重反應監測和 Protein-AQUA 驗證 RNAi 敲除技術

本討論將強調其中一些方法，即使用多重反應監測 (MRM) 和 Protein-AQUA。

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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