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T5569

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

同義詞:

TMB substrate for ELISA (Super Slow)

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

product name

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA, peroxidase substrate

形狀

liquid

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

3,3′,5,5′-Tetramethylbenzidine (TMB) is an electrochemical substrate for low level detection of horse radish peroxidase (HRP). It is a highly sensitive and dual function substrate, since it produces both soluble and insoluble reaction products. It has rapid reaction rate and it is suitable for on-line kinetic analysis. It does not possess carcinogenic properties.

應用

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA has been used as a substrate for enzyme linked immunosorbent assay (ELISA).
Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 60% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Pan-CC chemokine neutralization restricts splenocyte egress and reduces inflammation in a model of arthritis
Buatois V, et al.
Journal of Immunology, 185(4), 2544-2554 (2010)
Enzyme-linked Immunosorbent Assay (ELISA): From A to Z, 39-40 (2017)
Soluble amyloid-beta buffering by plaques in Alzheimer disease dementia versus high-pathology controls
Esparza TJ, et al.
PLoS ONE, 13(7), e0200251-e0200251 (2018)
Shiran Su et al.
PloS one, 17(10), e0276107-e0276107 (2022-10-19)
The blood-brain barrier (BBB) presents a major obstacle in developing specific diagnostic imaging agents for many neurological disorders. In this study we aimed to generate single domain anti-mouse transferrin receptor antibodies (anti-mTfR VHHs) to mediate BBB transcytosis as components of
Roberto Lande et al.
Antibodies (Basel, Switzerland), 9(2) (2020-05-15)
Human cathelicidin LL37 is a cationic antimicrobial peptide active against bacteria and viruses and exerting immune modulatory functions. LL37 can be also a target of autoreactive B- and T-lymphocytes in autoimmune settings. Irreversible post-translational modifications, such as citrullination and carbamylation

文章

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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