跳轉至內容
Merck
全部照片(1)

文件

STR1

Sigma-Aldrich

增强型禽类第一链合成试剂盒

Components for cDNA synthesis with enhanced AMV reverse transcriptase

同義詞:

eAMV-RT, 逆转录酶 cDNA 合成试剂盒

登入查看組織和合約定價
全部照片(1)

About This Item

分類程式碼代碼:
12352200
NACRES:
NA.55

用途

sufficient for 50 reactions
 kit sufficient for 50 reactions

特點

dNTPs included
hotstart: no

技術

RT-PCR: suitable

顏色

colorless

輸入

purified RNA

運輸包裝

dry ice

儲存溫度

−20°C

相關類別

一般說明

Sigma的cDNA第一链合成试剂盒（增强型禽类AMV-RT）还提供随机九核苷酸(9-mers)引物和anchored oligo(dT)23引物，以防有时基因特异性引物无法起效。anchored oligo由23个胸苷(T)残基和一个G、C或A残基（anchor/锚点）组成。锚点用于保证oligo(dT)引物结合在信使mRNA最起头处，不会留出一长段的未用序列。该试剂盒可产出适合各种下游应用的可靠cDNA，包括PCR。
cDNA第一链合成试剂盒（增强型鸟类AMV-RT）采用高度纯化的鸟类成髓细胞性白细胞病毒逆转录酶(eAMV-RT)，与标准AMV-RT或标准莫洛尼鼠类白血病病毒逆转录酶(MMLV-RT)相比，性能更加优越。 这种更加强大的eAMV逆转录酶可在高温（高达65℃）下转录棘手的二级结构，从总RNA或poly(A)+ RNA生成高质量的全长cDNA。

應用

cDNA第一链合成试剂盒（增强型禽类AMV-RT）可用于cDNA合成，以便用于实时逆转录-聚合酶链式反应(RT-PCR)。

單位定義

一单位酶可以多聚腺苷酸为模板，以12-18长度的oligo(dT)为引物，在10分钟内将1纳摩TMP整合进TCA-沉淀材料中。

法律資訊

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

僅套裝組件

產品號碼
描述
  • Enhanced Avian Reverse Transcriptase 1000 U

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • Deoxynucleotide mix 50 μL
  • O4387Anchored oligo (dT)23 100 μLSDS
  • R7647Random nonamers 100 μLSDS
  • O4387Ribonuclease inhibitor 50 μLSDS
  • W1754PCR grade water 1.5 mLSDS

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Aerosol-administered α-tocopherol attenuates lung inflammation in rats given lipopolysaccharide intratracheally.
Experimental Lung Research, 31, 34510-34524 (2007)
Sigma-Aldrich Corporation's Life Science Quarterly. Hot Start RT-PCR results in improved performance of the enhanced Avian RT-PCR
Easlund, E., and Mueller, E.
Sigma data, 2-5 (2001)
Heat shock proteins expression during thermal risk exposure in the temperate xerothermic ant Formica cinerea
Slipinski P, et al.
Sociobiology, 62(3), 457-459 (2015)
Julio Carrion et al.
Journal of immunology (Baltimore, Md. : 1950), 189(6), 3178-3187 (2012-08-15)
The low-grade oral infection chronic periodontitis (CP) has been implicated in coronary artery disease risk, but the mechanisms are unclear. In this study, a pathophysiological role for blood dendritic cells (DCs) in systemic dissemination of oral mucosal pathogens to atherosclerotic
JianFei Wang et al.
Journal of leukocyte biology, 82(6), 1554-1563 (2007-11-28)
Wound healing is a complex process involving the integrated actions of numerous cell types, soluble mediators, and ECM. Recently, a newly identified cell type, the fibrocyte, has been reported to contribute to wound healing and fibrotic conditions such as hypertrophic
查看所有相關論文

文章

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

反向轉錄

基因表達分析的挑戰包括 mRNA 的穩定性、時間性轉錄模式以及 mRNA 與蛋白質的相關性，這些都會影響準確性。

條款

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

相關內容

PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

PCR 應用

聚合酶連鎖反應 (PCR) 是一種擴增核酸分子的技術，常用於 RT-PCR、熱起始 PCR、終點 PCR 等多種應用。

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

查看全部

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務