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一般說明
锚定寡核苷酸(dT)23引物用于启动聚(A)尾的mRNA，以进行cDNA合成。引物有23个胸腺嘧啶残基，在3′端有一个G、C或A残基（锚点）。这个锚点可确保寡核苷酸(dT)引物在信使的起点结合，并且没有很长的不可用序列区域。增强型AMV逆转录酶、模板RNA、引物的最佳浓度和扩增参数取决于所使用的系统，并且应该根据经验确定。
應用
锚定寡核苷酸23 引物由23个胸腺嘧啶核苷残基组成，并在3′端具有一个G、C或A残基（锚）。该锚定位点可确保寡核苷酸(dT)23引物与mRNA起始端结合，直接跳过无用的长序列区域。以poly(A)+ RNA为模板制备cDNA时，该锚定寡核苷酸 (dT)23 引物比标准寡核苷酸 (dT) 引物更具优势。
特點和優勢
- 在制备cDNA文库过程中，当序列信息不完整或缺失或者特异性引物不合适时，可以使用Oligo(dT)23锚定引物和随机九聚体（产品编号R 7647）。
- 这些引物可以在cDNA第一链合成、cDNA文库构建和各种其他应用中作为逆转录引物的替代品。
- 转录后，oligo(dT)23锚定引物在高温（高达65 °C）下启动引物的能力降低，避免干扰PCR。
- 由poly(A)+ RNA产生cDNA时，oligo(dT)23锚定引物优于oligo(dT)标准引物。
其他說明
仅供实验室使用。不可用于药物、家庭或其他用途。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
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分析證明 (COA)
Lot/Batch Number
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文章
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
基因表達分析的挑戰包括 mRNA 的穩定性、時間性轉錄模式以及 mRNA 與蛋白質的相關性，這些都會影響準確性。
條款
The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.
相關內容
The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.
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