SRP6472

Transferrin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• 同義詞: DKFZp781D0156, PRO1557, PRO2086, TF
• 分類程式碼代碼: 12352202
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in HEK 293 cells
• 籤條: 6-His tagged (C-terminus)
• 化驗: ≥95% (SDS-PAGE)
• 形狀: lyophilized
• 分子量: calculated mol wt 76 kDa; observed mol wt 74 kDa by SDS-PAGE (DTT-reduced.)
• 包裝: pkg of 100 μg; pkg of 20 μg
• 雜質: <1 EU/μg endotoxin (LAL test)
• UniProt登錄號: P02787
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... TF(7018)

描述

一般說明

Transferrin is encoded by the TF gene that is mapped to human chromosome 3q22.1. It is a glycoprotein containing homologous iron-binding domains at N-terminal and C-terminal ends. Transferrin has a molecular weight of 80,000 Da and belongs to the transferrin superfamily, the members of which include lactoferrin, melanotransferrin, and ovotransferrin.

應用

To supplement neurosphere culture medium and sphere differentiation medium.

生化／生理作用

Transferrin is an iron-binding glycoprotein that primarily functions in the transport of plasma iron in vertebrates. It may also have a secondary function of facilitating cell proliferation. The binding of ferric iron to transferrin requires the presence of an anion, that functions as a bridge between the metal and the protein.

外觀

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

重構

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3