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重要文件

T1147

Sigma-Aldrich

脱铁转铁蛋白 人

apo-Transferrin human

powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)

同義詞:

人转铁蛋白, 铁传递蛋白

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.75
暫時無法取得訂價和供貨情況

生物源

human plasma

品質等級

產品線

BioReagent

化驗

≥98% (agarose gel electrophoresis)

形狀

powder

分子量

76-81 kDa

濃度

~25 mM

技術

cell culture | mammalian: suitable

雜質

HIV and HBsAg, source material tested negative
≤0.005% iron

溶解度

H2O: 50 mg/mL

UniProt登錄號

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... TF(7018)

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相關類別

應用

载脂蛋白转铁蛋白可以补充铁或用于结合培养基中存在的游离铁,还可用于将铁运输到哺乳动物细胞中。

生化/生理作用

转铁蛋白是一种存在于血清中的主要铁结合和细胞传递分子。 无血清细胞培养系统需要一种铁的传递形式。 转铁蛋白是铁的优选传递形式,因为细胞可通过细胞表面上的转铁蛋白受体以生理学上适当的方式对转铁蛋白结合的铁进行处理。 人载脂蛋白-转铁蛋白是一种高亲和力的转铁蛋白,可用于多种细胞类型。 载脂蛋白-转铁蛋白可以在使用前装载铁或直接添加到含铁介质中。

其他說明

非血红素铁转运蛋白。

免責聲明

供研究使用在法国,在用于科研目的时(包括进口和出口活动(《公共健康法》第L 1211-1条,第2节))该产品为管制品。购买者(即最终用户)需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


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存取文件庫

Giang D Nguyen et al.
PloS one, 8(8), e72698-e72698 (2013-08-24)
Huntington's disease (HD) is a neurodegenerative disease caused by abnormal polyglutamine expansion in the huntingtin protein (Htt). Although both Htt and the HD pathogenic mutation (mHtt) are implicated in early developmental events, their individual involvement has not been adequately explored.
Giang D Nguyen et al.
PloS one, 9(5), e96858-e96858 (2014-05-08)
H1 linker histone proteins are essential for the structural and functional integrity of chromatin and for the fidelity of additional epigenetic modifications. Deletion of H1c, H1d and H1e in mice leads to embryonic lethality by mid-gestation with a broad spectrum
Kathleen N O'Guin et al.
ASN neuro, 6(1), e00135-e00135 (2014-01-31)
The molecular requirements for human myelination are incompletely defined, and further study is needed to fully understand the cellular mechanisms involved during development and in demyelinating diseases. We have established a human co-culture model to study myelination. Our earlier observations
Kamil Strzelak et al.
Analytica chimica acta, 995, 43-51 (2017-11-12)
The Multicommutated Flow Analysis (MCFA) system for the estimation of clinical iron parameters: Serum Iron (SI), Unsaturated Iron Binding Capacity (UIBC) and Total Iron Binding Capacity (TIBC) has been proposed. The developed MCFA system based on simple photometric detection of
William J Buchser et al.
PloS one, 7(6), e38101-e38101 (2012-06-16)
Trauma to the spinal cord and brain can result in irreparable loss of function. This failure of recovery is in part due to inhibition of axon regeneration by myelin and chondroitin sulfate proteoglycans (CSPGs). Peripheral nervous system (PNS) neurons exhibit

文章

转铁蛋白和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统(用于生物制造包括单克隆抗体在内的异源蛋白)的性能。本页介绍转铁蛋白的体外化学和生物化学。

Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

Questions

1–3 of 3 Questions  
  1. Is this product sterile and can be used directly in cell culture media or does it have to be filtered first?

    1 answer
    1. This product is suitable for cell culture. Aqueous solutions of apo-transferrin should be stable at 4°C for 5–10 days. Solutions should be filter-sterilized for maximum stability. For more information, please refer to the product information sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/136/004/t1147pis.pdf

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  2. What is the carbohydrate content of human transferrin?

    1 answer
    1. We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  1. Cambridge UK
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    Cell culture supplement

    Supports neural stem and progenitor cell maintenance.

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