SRP3171

TGF-beta 3-E. coli human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 同義詞: Transforming Growth Factor-beta3
• 分類程式碼代碼: 12352202
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 形狀: lyophilized
• 效力: ≤0.05 ng/mL ED₅₀
• 分子量: 25.0 kDa
• 包裝: pkg of 10 μg
• 技術: cell culture | mammalian: suitable
• 雜質: <0.1 EU/μg endotoxin, tested
• 顏色: white
• UniProt登錄號: P10600
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... TGFB3(7043)

描述

一般說明

The gene TGF-β3 (transforming growth factor β 3) is mapped to human chromosome 14q24.3.[1] The three mammalian isoforms of TGF-β, TGF-β1, β2, β3 elicit similar biological responses.[2] TGF-β proteins are made as precursor molecules which are cut to generate a mature carboxy-terminal region of 110-140 amino acids.[3] They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1.[3][4] Recombinant TGF-β3 is a 25.0kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

應用

TGF-β3 (transforming growth factor β 3) human has been used in the media for the chondrogenic differentiation of the stromal cells.[5][6] It has been used in the culture medium to allow attachment of primary human dermal fibroblasts to the scaffolds.[7]

生化／生理作用

The three mammalian isoforms of TGF-β (transforming growth factor β), TGF-β1, β2, β3, are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing.[3] TGF-β3 is the predominant TGF-β in the testis and controls the blood-testis barrier activity. Polymorphisms in this gene might be associated with male infertility.[8] Heterozygous mutation in the gene at position Arg300 might be associated with increased TGF-β signaling, leading to a disorder with phenotypic overlap with Marfan syndrome and Loeys-Dietz syndrome.[9] TGF-β3 is involved in the mesenchymal stem cells (MSCs)-mediated inhibition of fibroblast proliferation and decreases skin fibrosis.[10]

序列

ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS

外觀

Lyophilized from 0.1% TFA

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 °C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 °C to -80 °C.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable