No. The cloning site, EcoR1, is ablated during production to prevent cloning into the vector. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/396/419/shc003bul.pdf
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一般說明
MISSION ® TurboGFP ™ 对照载体是一个 8347 碱基对的慢病毒质粒载体，含有编码 TurboGFP ™ 的基因，由 CMV 启动子控制。TurboGFP ™ 对照载体在使用 MISSION ® shRNA 文库克隆的实验中作为阳性转染/转导对照十分有用。[1][2] TurboGFP ™ 是从桡足类 Pontellina plumata 中克隆的绿色荧光蛋白 copGFP 的改良变体。
氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外，通过与相容的包装质粒共转染，可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒。TurboGFP 对照载体以 10μg 质粒 DNA，溶于 Tris-EDTA (TE) 缓冲液中，浓度为 500 ng/μl.
氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外，通过与相容的包装质粒共转染，可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒。TurboGFP 对照载体以 10μg 质粒 DNA，溶于 Tris-EDTA (TE) 缓冲液中，浓度为 500 ng/μl.
應用
MISSION® pLKO.1-puro-CMV-TurboGFP ™ 阳性对照质粒 DNA 已被用于构建 GATA2 过表达载体 (oeG2)，方法是在 5′ 端去掉该载体的 GFP 盒。NheI 和 3′PstI 克隆位点，并用全长 GATA2 转录序列替代。[3]
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna
法律資訊
使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
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產品號碼
描述
訂價
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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文章
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
當使用慢病毒載體傳遞 shRNA 時，編碼 shRNA 的序列會整合到基因組中，而基因敲除的效果會傳給子細胞，繼續基因沉默。
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dear officer I'd like to know if I'm able to clone in shRNA of choice downstream of the U6 promoter of this plasmid (SHC003)? is there any RE site available to do so? Thank you
1 answer-
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Can I purchase lentiviral particles produced using MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003, directly from Sigma-Aldrich?
1 answer-
Yes. The product code is SHC003V.
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What competent cells can I use to propagate the MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003?
1 answer-
We use GC5 cells (identical genotype to DH5α-T1R) to propagate the Mission shRNA plasmids and controls.
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How much plasmid DNA do I receive when I order MISSION® Control Vector Purified DNA TurboGFP™, Product SHC003?
1 answer-
Each MISSION Control Vector is provided as 10 μg purified plasmid DNA at a concentration of ~500 ng/μL in 10 mM Tris-HCl, pH 8.0, containing 1 mM EDTA.
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Can I use MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003, to produce lentiviral particles?
1 answer-
The MISSION® Lentiviral Packaging Mix can be used if you wish to produce virus in your own lab. The product code is SHP001. This product is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene. The Lentiviral Packaging Mix is designed to be co-transfected along with a compatible lentiviral transfer vector (such as SHC003) into HEK293T cells in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. The procedure is available in the Technical Bulletin for product SHP001.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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