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SHC003V

MISSION^®

Name: MISSION<SUP>®</SUP>
Brand: SIGMA

Green fluorescent protein marker to monitor transduction efficiency

同義詞:

MISSION^®, MISSION^® TurboGFP^™ Control Transduction Particles

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About This Item

分類程式碼代碼:

41106609

NACRES:

NA.51

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SHC003

MISSION^® pLKO.1-puro-CMV-TurboGFP^™ 阳性对照质粒 DNA

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SHC002V

MISSION^®

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SHC001

MISSION^®pLKO.1-puro空载体对照质粒 DNA

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SHCLNV

MISSION^® shRNA

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SHP001

MISSION ^® 慢病毒包装混合物

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SHC004

MISSION^® 对照载体

SHRNA

MISSION^® shRNA Product Offerings

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SHC004V

MISSION^® TurboGFP^™ shRNA Control Transduction Particles

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SHC016

MISSION^® pLKO.1-puro 非靶 shRNA 对照质粒DNA

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SHC012V

MISSION^®

品質等級

100

產品線

MISSION^®

濃度

≥1x10⁶ VP/ml (via p24 assay)

技術

capture ELISA: 10⁶ TU/mL using p24

運輸包裝

dry ice

儲存溫度

−70°C

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一般說明

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP^™ is an improved variant of the green fluorescent protein copGFP cloned from the copepoda Pontellina plumata. The TurboGFP^™ transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP^™ (SHC003). It is a positive control to monitor transduction efficiency.

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 10⁶ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

当使用MISSION^® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。

應用

MISSION^® pLKO.1-puro-CMV-TurboGFP^™ Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.

法律資訊

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TurboBeads is a trademark of TurboBeads LLC

TurboGFP is a trademark of Evrogen Co.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析證明 (COA)

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文章

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

提高慢病毒轉導效率的方法

比較了慢病毒轉導 Jurkat 細胞的方法。比較了 Spinoculation 與 polybrene (hexadimethrine bromide) 及 fibronection 塗佈板的過夜孵育。

條款

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

慢病毒轉導協議

如何進行 MISSION shRNA 慢病毒顆粒的慢病毒轉導，以達到長期穩定的沉默和表型改變的詳細程序。

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

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文件

MISSION®

Green fluorescent protein marker to monitor transduction efficiency

About This Item

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屬性

品質等級

產品線

濃度

技術

運輸包裝

儲存溫度

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描述

一般說明

應用

法律資訊

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安全資訊

儲存類別代碼

水污染物質分類（WGK）

閃點（°F）

閃點（°C）

個人防護裝備

文件

分析證明 (COA)

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MISSION^®