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重要文件

RAB0874

Sigma-Aldrich

猪INS /胰岛素ELISA试剂盒

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About This Item

分類程式碼代碼:
41116158
NACRES:
NA.32
暫時無法取得訂價和供貨情況

物種活性

porcine

包裝

kit of 96 wells (12 strips x 8 wells)

技術

ELISA: suitable

輸入

sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 μIU/mL
standard curve range: 4.69 μIU/ml-300 μIU/ml

檢測方法

colorimetric

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

Porcine ... INS(397415)

一般說明

The antibody reacts specifically against insulin by RIA and immunocytochemistry.[1][2][3] It exhibits cross-reactivity with human proinsulin.[3] The antibody binds to insulin with an affinity constant of 8.8 × 109 M-1 in RIA.[1][2] The insulin gene is mapped to human chromosome 11p15.5.[4]
Human insulin is made up of 51 amino acids. It consists of two peptide chains, 21 amino acids make up A-chain and chain B contains 31 amino acids. The two chains are linked together by a disulfide bond, to form a classical insulin fold with three α helices, chain A having two and one in chain B.[5]

應用

请参考Protocol了解详情。

生化/生理作用

Abnormal insulin secretion or function, leads to diabetes mellitus, obesity and other carbohydrate metabolism disorders.[6][7] Insulin is known to stimulate cell division. The biological effects of insulin are mediated by insulin receptor and it integrates major signaling pathways.[6]

其他說明

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

象形圖

Corrosion

訊號詞

Warning

危險聲明

防範說明

危險分類

Met. Corr. 1

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

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存取文件庫

Proinsulin-Specific Monoclonal Antibodies: Immunocytochemical Application as ?-Cell Markers and as Probes for Conversion.
Madsen O D
Diabetes, 36(10), 1203-1211 (1987)
Genetic linkage of Beckwith-Wiedemann syndrome to 11p15.
Ping A J, et al.
American Journal of Human Genetics, 44(5), 720-720 (1989)
Kinetic properties of monoclonal insulin antibodies.
Keilacker H, et al.
Biomedica Biochimica Acta, 45(8), 1093-1102 (1986)
S Witt et al.
Acta histochemica. Supplementband, 35, 217-223 (1988-01-01)
Murine monoclonal antibodies against glucagon and insulin were generated by somatic cell hybridization and partially characterized. The monoclonal glucagon antibody K79bB10 exhibited no cross-reaction with gut glucagon. This antibody and the insulin antibody K36aC10 were found of very high concentration
Metabolic endotoxemia initiates obesity and insulin resistance.
Cani P D, et al.
Diabetes, 56(7), 1761-1772 (2007)

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