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重要文件

RAB0026

Sigma-Aldrich

人类BNDF脑源性营养因子ELISA试剂盒

for serum, plasma, cell culture supernatant and urine

同義詞:

BDNF, Brain-derived neurotrophic factor

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About This Item

分類程式碼代碼:
41116158
NACRES:
NA.32
暫時無法取得訂價和供貨情況

物種活性

human

包裝

kit of 96 wells (12 strips x 8 wells)

技術

ELISA: suitable
capture ELISA: suitable

輸入

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 0.066-16 ng/mL

檢測方法

colorimetric

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... BDNF(627)

一般說明

人 BDNF ELISA(酶联免疫吸附试验)试剂盒是一种体外酶联免疫吸附试验,用于定量测定血清、血浆、细胞培养上清液和尿液中的人 BDNF。
脑源性神经营养因子(BDNF)基因定位于人类染色体 11p14.1 上。[1]BDNF是生长因子中神经营养蛋白家族的成员,该 [2] 该基因编码前体蛋白proBDNF。成熟的BDNF(mBDNF)是通过proBDNF的翻译后切割合成的。proBDNF 和 mBDNF 在细胞信号传导中都起着至关重要的作用。[3]

免疫原

重组人 BDNF

應用

人BDNF ELISA试剂盒已用于:
  • 测定脑源性神经营养因子(BDNF)蛋白表达[4]
  • 测定脑片培养基和器官型脑片培养(OBSC)提取物中的BDNF水平[5]
  • 测定血浆BDNF水平[6]

仅供研究使用。用于诊断程序。
人 BDNF ELISA 试剂盒已用于确定骨髓间充质干细胞 (MSC) 移植对 Tourette 综合征 (TS) 大鼠纹状体中 BDNF 蛋白表达的影响。[7] 也用于测定 GD1(高雪氏病 1 类型)患者和对照人群的血浆 BDNF 水平。[8]

生化/生理作用

脑源性神经营养因子(BDNF)参与人类的海马功能和言语情节记忆。因此,BDNF 基因表达的变异会改变这些神经功能。[9]BDNF在血管功能中也起着至关重要的作用,并通过特异性受体原肌球蛋白相关激酶B(TrkB)参与血管生成。[10]它参与了阿尔茨海默′病的发病机制。ProBDNF 与 p75 神经营养因子受体相互作用,导致海马体的长期抑制。[3]

其他說明

本产品提供样本检验报告。
请在批号对应的文本框中输入样品 一词。

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

象形圖

Corrosion

訊號詞

Warning

危險聲明

防範說明

危險分類

Met. Corr. 1

儲存類別代碼

8A - Combustible corrosive hazardous materials


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分析證明 (COA)

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存取文件庫

Oxidative Stress-Tolerant Stem Cells from Human Exfoliated Deciduous Teeth Decrease Hydrogen Peroxide-Induced Damage in Organotypic Brain Slice Cultures from Adult Mice
Xiao L, et al.
International Journal of Molecular Sciences, 20(8), 1858-1858 (2019)
Xiumei Liu et al.
Experimental and therapeutic medicine, 11(4), 1211-1216 (2016-04-14)
The aim of the present study was to investigate the effect of bone marrow mesenchymal stem cell (MSC) transplantation on brain-derived neurotrophic factor (BDNF) expression in the striatum of Tourette syndrome (TS) rats. In addition, the possible mechanism of MSC
Hélio José Coelho-Júnior et al.
International journal of environmental research and public health, 17(10) (2020-05-20)
The present study compared the effects of traditional resistance training (TRT) and combined power training (PT) and TRT (PTRT) on cognitive parameters and serum brain-derived neurotrophic factor (BDNF) levels in non-demented, well-functioning, community-dwelling older women. Forty-five older women were randomized
Shervin Pejhan et al.
Frontiers in cell and developmental biology, 8, 763-763 (2020-09-26)
Rett Syndrome (RTT) is a rare and progressive neurodevelopmental disorder that is caused by de novo mutations in the X-linked Methyl CpG binding protein 2 (MECP2) gene and is subjected to X-chromosome inactivation. RTT is commonly associated with neurological regression
Effect of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome
Liu X, et al.
Experimental and Therapeutic Medicine, 11(4), 1211-1216 (2016)

Questions

  1. Could you please confirm if this kit is capable of recognizing both free and bound BDNF?

    1 answer
    1. Epitope mapping has not been conducted, so the exact binding site is unknown. However, the immunogen is located at aa128-247 (Accession# P23560).

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