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PKH26GL

Sigma-Aldrich

用于常规细胞膜标记的PKH26红色荧光细胞连接子试剂盒

Distributed for Phanos Technologies

同義詞:

Red PKH membrane dye

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.32
暫時無法取得訂價和供貨情況

包裝

pkg of 1 kit

品質等級

200

製造商／商標名

Distributed for Phanos Technologies

儲存條件

protect from light

螢光

λex 551 nm; λem 567 nm (PKH26 dye)

檢測方法

fluorometric

運輸包裝

ambient

儲存溫度

room temp

相關類別

一般說明

PKH26是一种红色荧光染料。这种亲脂性的膜染料被广泛用于通过流式细胞术对异质性细胞中的细胞增殖进行测定。[1]

應用

本试剂盒适用于常规细胞膜标记。本试剂盒已用于鉴定一系列模式系统并适用于体外细胞标记，体外增殖研究以及长期的体内细胞跟踪研究。标记在兔红细胞上的PKH26半衰期长达100天以上。增强的稳定性适用于长期的体内研究。
用于常规细胞膜标记的PKH26红色荧光细胞连接子试剂盒。它已在多种模型系统中得到表征，并已被发现可用于体外细胞标记、体外增殖研究和长期的体内细胞示踪。[2][3]来自标记的兔红细胞的PKH26洗脱液的半衰期大于100天。这种增强的稳定性对于长期的体内研究是有利的。

聯結

如需关于PKH及CellVue®的荧光细胞连接子染料的更多技术详情，包括大量的参考书目，请访问这里。

法律資訊

CellVue is a registered trademark of Phanos Technologies

僅套裝組件

產品號碼
描述
  • Diluent C 6 x 10
  • PKH26 Cell Linker in ethanol .5 mL

相關產品

產品號碼
描述
訂價

象形圖

FlameExclamation mark
GHS02,GHS07

訊號詞

Danger

危險聲明

H225,H319

危險分類

Eye Irrit. 2 - Flam. Liq. 2

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

57.2 °F - closed cup

閃點（°C）

14.0 °C - closed cup

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
MKCV7397
MKCV4102
MKCV7789
MKCR6075
MKCQ6477

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文章

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

用於螢光細胞標記的 PKH 連接劑試劑盒

PKH 染料使用方便，可實現活細胞的穩定、均勻和可重複的螢光標記。PKH 染料是無毒的膜染色劑，可產生高信噪比。

Lipophilic Dye Kits for Cell Tracking

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

用於細胞追蹤的親脂染料試劑盒

親油性細胞追蹤染料可讓癌症生物學家在體外和體內追蹤腫瘤和免疫細胞的功能。閱讀文章，選擇合適的膜染料試劑盒進行細胞追蹤和增殖監測。

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Questions

1–10 of 10 Questions  

  1. If we want to use the PKH to label the membrane of fibroblast, and then co-culture them with tumor cells in order to interact, ¿ Will PKH transfer from fibroblast to tumor cells?

    1 answer

    1. PKH dyes are routinely used in co-culture situations. Because staining is nearly instantaneous, rapid and homogeneous dispersion of cells in dye solution is essential. There should be no excess or unincorporated dye in the medium prior to mixing or co-culturing the cell lines. Free dye will be taken up by all cells. See the links below to the product protocol as well as two additional co-culture studies using PKH dyes:

      Protocol
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf

      Co-culture in scaffolds
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155425/

      Co-culture, mixed monolayer, and on Transwell
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5168417/

      Helpful?

  2. Are cells lost during the PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling staining process?

    1 answer

    1. Over-labeling of the cells will result in loss of membrane integrity and reduced cell recovery.  Methods for improving cell viability can be found on the troubleshooting guide.

      Helpful?

  3. When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?

    1 answer

    1. Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.

      Helpful?

  4. What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?

    1 answer

    1. A protocol for visualization of tissue sections can be found in the technical bulletin.  For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells.  If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.

      Helpful?

  5. Will Product PKH26GL, PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling Products, stain dead cells?

    1 answer

    1. As long as the cell has an intact membrane, the PKH26 dye can label the cell.

      Helpful?

  6. What is the difference between PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling Products PKH26GL and PKH26PCL?

    1 answer

    1. The general labeling kit (Product No. PKH26GL) will label the membrane of the cells.  For phagocytic cells, Product No. PKH26PCL is used.  This kit utilizes dye aggregates for specific labeling of phagocytic cells in the presence of other cell types.

      Helpful?

  7. Does the dye leak from the cells after labeling when using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling?

    1 answer

    1. In general, slow loss of fluorescence has been observed with PKH1 and PKH2 in in vivo studies and it is likely that PKH67 may also slowly lose fluorescence. Leakage (or loss of staining intensity) may also occur due to cell-to-cell transfer of the dye. The most common cause of cell-to-cell dye transfer is inadequate washing of the cells. Wash cells 3-5 times after labeling and transfer samples to new tubes between washes.

      Helpful?

  8. Product PKH26GL, PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, is a red dye.  Do you offer a kit that stains the cells green?

    1 answer

    1. We offer Product numbers:PKH2GL Both of these kits stain the cells with a dye that emits in the green fluorescent range.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. How many cells can be stained with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Products PKH26GL and MINI26?

    1 answer

    1. For Product PKH26GL, when using the concentrations indicated in the product bulletin, you can stain 30 samples (1x107 cells/sample).The kit provides 500 ul of PKH26 dye solution.  You use 4 ul per sample.  There is enough dye in the kit to stain 125 samples.The kit provides 60 mls of Diluent C.  You use 2 mls per sample (1 ml for dilution of the dye, 1 ml for dilution of the cells).  There is enough diluent for 30 samples.You can purchase additional diluent as Product CGLDILIf you need less dye, you can purchase Product MINI26 which contains 100 ul of dye and 10 mls of Diluent C.  This kit contains enough dye to stain 25 samples and enough diluent to stain 5 samples.

      Helpful?

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