It is recommended to fix and permeabilize the cells before staining with the BioTracker membrane dye and prior to performing immunofluorescent staining. To prevent dye redistribution, avoid using detergents after the staining step.
SCT107
BioTracker 555橙色细胞质膜染料
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
同義詞:
Lipophilic carbocyanine dye, Live cell imaging probe, Live cell membrane dye
About This Item
推薦產品
技術
cell based assay: suitable
檢測方法
fluorometric
運輸包裝
ambient
一般說明
与PKH染料不同,BioTracker细胞质膜染料不需要复杂的低渗标记方案,它们是即用型染料递送溶液,可直接加入正常培养基中以标记悬浮或粘附的培养细胞。
光谱性质
吸光度: 549nm
发射:565nm
應用
活细胞染料
细胞成像
品質
吸光度: 549nm
发射:565nm
外觀
儲存和穩定性
注意:打开小瓶前,短暂离心小瓶以收集小瓶底部的内容物。
免責聲明
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Flam. Liq. 2
儲存類別代碼
3 - Flammable liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
55.4 °F - closed cup
閃點(°C)
13 °C - closed cup
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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If using this dye on paraformaldehyde-fixed cells alongside antibody-based immunofluorescent staining, when should membrane labeling be performed?
1 answer-
Helpful?
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In order to stain fixed cells, do we have to use PBS or other buffer at room temperature instead of culture medium at 37oC like Biotracker 400 Blue Cytoplasmic Membrane dye?
1 answer-
BioTracker 555 Orange Cytoplasmic Membrane Dye (Cat. no. SCT107) is a lipophilic carbocyanine dye. Lipophilic carbocyanine dyes like this product have also been used to stain cells or tissues after formaldehyde fixation.
For a detailed protocol of the assay, please see the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/876/849/sct107ds.pdfHelpful?
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