The MINI67 kit includes enough PKH67 dye for 25 samples and enough Diluent C for 10 samples, assuming a 1 mL sample size. Additionally, the MINI67 kit with PKH67 dye is designed to stain 125 samples. It is important to note that actual results may vary depending on cell type, among other factors.
packaging
pkg of 1 kit
Quality Segment
manufacturer/tradename
Distributed for Phanos Technologies
storage condition
protect from light
technique(s)
flow cytometry: suitable, live cell imaging : suitable
fluorescence
λex 490 nm; λem 502 nm (PKH67 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
General description
PKH67试剂盒用于常规细胞膜标记。它具有比PKH1和PKH2（之前在体外和体内细胞示踪应用体积的另外两种绿色染料）更长的脂肪族碳尾。基于更长的尾，内部研究一致表明与PKH2相比，PKH67的细胞转移减少。
使用PKH1和PKH2进行的体内研究观察到荧光缓慢消失。PKH67可能表现出相似性质，因为这种行为似乎是绿色细胞接头染料的特征，而非红色细胞接头染料的特征。 非分裂期细胞的体外细胞膜的保留性质与体内荧光半衰期之间的相关性，预测PKH67的体内荧光半衰期为10-12天。 其他半衰期相似的绿色细胞接头染料已用于监测1-2个月内体内淋巴细胞和巨噬细胞运输，表明PKH67还可用于中等长度的体内示踪研究。
使用PKH1和PKH2进行的体内研究观察到荧光缓慢消失。PKH67可能表现出相似性质，因为这种行为似乎是绿色细胞接头染料的特征，而非红色细胞接头染料的特征。 非分裂期细胞的体外细胞膜的保留性质与体内荧光半衰期之间的相关性，预测PKH67的体内荧光半衰期为10-12天。 其他半衰期相似的绿色细胞接头染料已用于监测1-2个月内体内淋巴细胞和巨噬细胞运输，表明PKH67还可用于中等长度的体内示踪研究。
Application
此试剂盒用于常规细胞膜标记。使用PKH1和PKH2进行的体内研究观察到荧光缓慢消失。PKH67可能表现出相似性质，因为这种行为似乎是绿色细胞接头染料的特征，而非红色细胞接头染料的特征。
Other Notes
更多关于PKH和CellVue® 荧光细胞连接染料的技术详情，包括丰富的文献资料，请访问 这里。
Legal Information
由Phanos Technologies, Inc.分发
CellVue is a registered trademark of Phanos Technologies
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本產品 | |||
|---|---|---|---|
| description Distributed for Phanos Technologies | description Distributed for Phanos Technologies | description Distributed for Phanos Technologies | description Distributed for Phanos Technologies |
| technique(s) flow cytometry: suitable | technique(s) - | technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition protect from light |
| manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename - | manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| application(s) cell analysis | application(s) - | application(s) cell analysis | application(s) cell analysis |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
僅套裝組件
產品號碼
描述
- Diluent C 10 mL
- PKH67 Cell Linker in ethanol 0.1 mL
警示詞
Danger
危險代碼
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
存儲類別/等級
3 - Flammable liquids
閃點 (F)
55.4 °F - closed cup
閃點（°C）
13 °C - closed cup
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how many assays can be obtained from the PKH67 green fluorescent cell linker mini kit? Additionally, approximately how many number of cells that can be labeled using the mini kit?
1 answer-
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