The long term stability of this product in solution is not been investigated. However, aqueous solutions are expected to be stable for at least 6 months when stored in working aliquots at -20°C. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/143/182/p9582pis.pdf
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應用
TLR3可识别双链RNA，是病毒病原体免疫应答的主要效应物。 聚肌苷酸-聚胞苷酸 (Poly(I) • Poly(C))是一种双链均聚物，可作为模型RNA，用于研究在TLR3水平下的细胞信号传导。 Poly(IC)是TRIF依赖型toll样受体3（TLR3）的配体。
生化／生理作用
将Poly(I:C)转染至NIT-1细胞中，已被用作细胞内dsRNA诱导的β细胞凋亡的模型。转染后18小时，45％的细胞发生凋亡，并且其NF-kB、p50/p65核转位、caspase 3和8切割以及caspase 12的转录诱导、Fas、IL-15和TNF受体相关配体（TRAIL）水平均增加。有人提出Poly(I:C)是最合适的稳定成熟树突状细胞（DC）诱导剂之一。这些成熟DC能够在CD40连接后分泌生物活性IL-12，因此注射后能够在体内产生有效的免疫应答。Poly(I:C)被用作有效的佐剂，以增强对肽类疫苗的特异性抗肿瘤免疫应答。
包裝
包装规格取决于多核苷酸含量
其他說明
双链均聚物。
準備報告
本品需要离子强度，以维持双链结构。用水复溶至浓度约为10 mg/mL，获得溶于生理磷酸盐缓冲溶液的多核苷酸。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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What is the stability of this product in solution? For example, how long would a 5 mg/mL solution last when stored at -20 celcius?
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What is the size (bp) of this product?
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This product has a estimated size range of 272 - 4242 bases with a resulting molecular weight range of 90,000 - 1,400,000 daltons.
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Does the product P9582-5MG contain 5mg of polyI:C or 500ug (10%) of poly I:C and 4.9mg buffer salts?
1 answer-
This product contains 10% Poly (I:C) with sodium chloride and sodium phosphate buffer salts. Please see the link below to a sample Certificate of Analysis for further details regarding concentration of the Poly (I:C).
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P9582-50MG0000213739.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How stable is Product P9582, Polyinosinic-polycytidylic acid potassium salt, in solution?
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RNA and synthetic RNA analogues will be very stable in aqueous solution as long as the solutions are not contaminated with RNase.
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What is the molecular weight of Product P9582, Polyinosinic-polycytidylic acid potassium salt?
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The molecular weight may vary from lot to lot with a range from 90,000 to 1,400,000 with an average of 200,000 to 500,000.
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Why is the total mass of Product P9582, Polyinosinic-polycytidylic acid potassium salt, in my bottle much greater than the designated package size?
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The package size is based on the polynucleotide content. However, since the polynucleotide is supplied with sodium chloride and sodium phosphate buffer salts, its mass represented by the package size is approximately 10% of the total mass present in the bottle.
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Should Polyinosinic-polycytidylic acid potassium salt Product P9582 or P1038 be used for cell activation?
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For cell activation, synthetic double-stranded RNA is widely used as ligand of Toll-like receptor 3 (TLR3) in place of what is supposed to be the natural ligand (viral dsRNA). The double stranded RNA homopolymer, i.e. Poly(I):Poly(C), Prod. No. P9582 can be used for this application. P1038 is a single-stranded RNA and will not activate TLR3.
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What is the difference between Polyinosinic-polycytidylic acid potassium salt Products P9582, P1038, P1530 and P0913?
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Prod No. P9582 as well as Prod. Nos. P1530 and P0913 are double-stranded with one strand of Poly(I) and one strand of Poly(C). Prod. No. P1038 is a single-stranded RNA analog with C and I randomly making up the polynucelotide strand. Double-stranded Prod. No. P9582 is a potassium salt while double-stranded Prod. Nos. P0913 and P1038 are sodium salts. Prod. No. P0913 is gamma-irradiated. None of the other products have been gamma-irradiated. Unlike the other products, only approximately 10% of the total mass of Prod. No. P9582 is actually polynucleotide. The remaining 90% is buffer salts as sodium chloride and sodium phosphate.
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