P7623

PKD2 Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), aqueous glycerol solution

• 同義詞: Protein Kinase D2
• MDL號碼: MFCD07371065
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 重組細胞: expressed in E. coli
• 品質等級: 200
• 化驗: ≥70% (SDS-PAGE)
• 形狀: aqueous glycerol solution
• 比活性: 100 units/mg protein (Bradford)
• 分子量: protein apparent mol wt ~140 kDa
• UniProt登錄號: Q9BZL6
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PRKD2(25865)

描述

應用

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBTIDE substrate peptide (KRREILSRRPSYR), per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

生化／生理作用

PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation.

單位定義

One unit will incorporate one nanomole of phosphate into the CREBTIDE substrate (KRREILSRRPSYR) per minute at 30 °C at pH 7.2 using a final concentration of 50 μM [32P] ATP.

外觀

Solution of 50 μl in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids