Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
暫時無法取得訂價和供貨情況
推薦產品
產品名稱
色氨酸羟化酶单克隆抗体 小鼠抗, clone WH-3, ascites fluid
生物源
mouse
共軛
unconjugated
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
WH-3, monoclonal
分子量
antigen 55 kDa
包含
15 mM sodium azide
物種活性
human, monkey, rabbit, rat
技術
immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using rabbit pineal gland extract
同型
IgG3
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... TPH1(7166) , TPH2(121278)
rat ... Tph1(24848) , Tph2(317675)
一般說明
单克隆抗色氨酸羟化酶（小鼠IgG3 亚型）来自于小鼠骨髓瘤细胞和BALB/c小鼠脾细胞融合产生的WH-3杂交瘤细胞。色氨酸羟化酶(TPH；L-色氨酸四氢蝶啶：氧气氧化还原酶；EC 1.14.16.4），用于合成血清素的限速酶，是一种只存在于含血清素的神经元中的特殊酶。
色氨酸羟化酶1基因（TPH1）是一个含444个氨基酸的蛋白质，由跨越29kbp的基因编码，位于具有11个外显子的人染色体11p15.3-p14上。该酶表达于肠、脾、胸腺、垂体以及松果体中。
特異性
该抗体可与色氨酸羟化酶发生特异性反应。
免疫原
重组兔色氨酸羟化酶。
應用
小鼠源抗色氨酸羟化酶单克隆抗体可用于：
- 酶联免疫吸附测定(ELISA)
- 免疫组织化学
- 免疫印迹
- 免疫细胞化学
生化／生理作用
色氨酸羟化酶1基因（TPH1）催化5-羟色氨酸的合成，而5-羟色氨酸是神经递质5-羟色胺的前体。基因表达的变化会影响反应抑制过程中前额叶皮层的激活。TPH1基因的突变会导致精神分裂症，女性肠易激综合征（IBS），自杀行为和抑郁症。编码的蛋白质在调节心血管功能中起着至关重要的作用。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
未找到適合的產品？
試用我們的產品選擇工具.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
客戶也查看了
Kadri Andre et al.
BMC psychiatry, 13, 118-118 (2013-04-20)
In major depression, one of the candidate genes possibly affecting the risk and severity of symptoms has been found to be tryptophan hydroxylase (TPH1). Variation in treatment response to antidepressive agents according to TPH1 genotype has also been found in
Honghai Zhang et al.
Epilepsia, 57(8), 1228-1235 (2016-06-16)
The DBA/1 mouse is a relevant animal model of sudden unexpected death in epilepsy (SUDEP), as it exhibits seizure-induced respiratory arrest (S-IRA) evoked by acoustic stimulation, followed by cardiac arrhythmia and death. Defects in serotonergic neurotransmission may contribute to S-IRA.
Brain tryptophan hydroxylase: purification of, production of antibodies to, and cellular and ultrastructural localization in serotonergic neurons of rat midbrain
Joh TH, et al.
Proceedings of the National Academy of Sciences of the USA, 72(9), 3575-3579 (1975)
Chao-Chuan Wang et al.
The international journal of neuropsychopharmacology, 16(9), 2027-2039 (2013-07-05)
Accumulating evidence suggests that dysfunction of the amygdala is related to abnormal fear processing, anxiety, and social behaviors noted in autistic spectrum disorders (ASDs). In addition, studies have shown that disrupted brain serotonin homeostasis is linked to ASD. With a
Anthony C Ruocco et al.
Neuropsychology, 30(1), 18-27 (2015-12-29)
The tryptophan hydroxylase 1 gene (TPH1) catalyzes the formation of 5-hydroxytryptophan, a precursor to the neurotransmitter serotonin. Variations in the gene encoding this enzyme may underlie difficulties in impulse control; however, the proximate relationship between risk alleles for polymorphisms in
-
What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
-
-
Do you have an immunohistochemistry protocol for use with Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse?
1 answer-
Free floating sections (4% paraformaldehyde fixation)1. Incubate 30 minutes at room temperature in 0.3% Triton X-100, 5% normal serum.2. Incubate with the antibody diluted 1:10,000 in PBS, 0.3% Triton X-100, 3.5% normal serum and 0.05% BSA for 36-48 hours.3. Rinse 3 times for 5 minutes each in PBS.4. Add the biotinylated anti-mouse IgG3 diluted in PBS containing 0.3% Triton X-100 and 3.5% normal serum for 1 hour at room temperature.5. Rinse 3 times for 5 minutes each in PBS.6. Process with Avidin Biotin-HRP.7. Complete the reaction with a 2-10 minute incubation in PBS containing 0.05% DAB and 0.015% hydrogen peroxide (H2O2).8. Mount the sections on gel coated slides and air dry.
Helpful?
-
-
Does Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, cross-react with tyrosine or phenylalanine hydroxylases?
1 answer-
This antibody, unlike some other antibodies to tryptophan hydroxylases (WH) in the market, does not cross react with tyrosine hydroxylase (TH) or with phenylalanine hydroxylase (PH). The epitope recognized by the antibody is not located in the Pterin Binding Region, which is homologous among PH, TH and WH. The specificity to WH is confirmed by the single band obtained at the 55KD region, while TH has a higher MW (60-68KD), depending on the submit) and PH has a lower MW. It has also been confirmed by collaborating experts. See Journal of Neuroscience Methods, 114(2), 205-212 (2002).
Helpful?
-
-
Can Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, be used to stain mouse brain sections?
1 answer-
This antibody has been used successfully on mouse brain slices in an immunohistochemical application. The IHC results from an experiment employing T0678 at 1:500 in mouse brain slice staining clearly showed the dorsal nuclear raphe (which are the serotonergic neurons).
Helpful?
-
Active Filters
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務
