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重要文件

P7348

Sigma-Aldrich

T7 Phage Promoter Primer Set

同義詞:

5′-TAA TAC GAC TCA CTA TAG GG-3′

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About This Item

分類程式碼代碼:
41106305
暫時無法取得訂價和供貨情況

儲存溫度

2-8°C

應用

T7 Phage Promoter Primer Set is a single-stranded oligonucleotide with 5′-hydroxyl and 3′-hydroxyl ends and a selection of four fluorescent lables for use in polymerase chain reaction protocols (PCR). Functionally tested for use in fluorescence-detection automated sequencing.

包裝

Packaged in amber tubes to protect from light.

成分

Set of four vials of primer, each individually labeled at the 5′-end with:
FAM: (5[6]-carboxyfluorescein)
JOE: (6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein)
ROX: (5[6]-carboxy-X-rhodamine), and
TAMRA: (5[6]-carboxytetramethylrhodamine.
Supplied as solutions, 50 μl each, in tris-EDTA buffer, pH 7.5, at 1 picomole/μl

分析報告

Tested for purity with HPLC, PAGE, and OD.

儲存類別代碼

10 - Combustible liquids


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Yajing Zhou et al.
PloS one, 6(7), e22224-e22224 (2011-07-27)
Eukaryotic DNA polymerase δ (pol δ) plays a crucial role in chromosomal DNA replication and various DNA repair processes. It is thought to consist of p125, p66 (p68), p50 and p12 subunits. However, rigorous isolation of mammalian pol δ from
High-throughput sequencing of PCR products tagged with universal primers using 454 life sciences systems.
Daigle D, Simen BB, Pochart P.
Current Protocols in Molecular Biology, Unit 7-Unit 7 (2011)
Jacquelina Williams-Woods et al.
Journal of virological methods, 178(1-2), 253-257 (2011-10-04)
Human norovirus (HuNoV) and hepatitis A (HAV) are recognized as leading causes of non-bacterial foodborne associated illnesses in the United States. DNA sequencing is generally considered the standard for accurate viral genotyping in support of epidemiological investigations. Due to the
Angela Capece et al.
International journal of food microbiology, 144(1), 187-192 (2010-10-12)
The present research studied Saccharomyces cerevisiae yeasts isolated from Nero d'Avola grapes, collected in different areas of the Sicily region. RAPD-PCR analysis with M13 primer was used for preliminary discrimination among 341 S. cerevisiae isolates. Inoculated fermentations with S. cerevisiae
K A Abd-Elsalam et al.
Genetics and molecular research : GMR, 9(4), 2016-2024 (2010-10-20)
Seven fungal isolates were identified as pan-global Hypocrea/Trichoderma species, from section Trichoderma, on the basis of their morphology. These species were H. lixii/T. harzianum and H. orientalis/T. longibrachiatum. PCR-based markers with primer M13 (core sequence of phage M13) and internal-transcribed

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