推薦產品
等級
for molecular biology
形狀
buffered aqueous glycerol solution
分子量
~80 kDa
濃度
≥10,000 units/mL
UniProt登錄號
異物活動
DNase, RNase, none detected
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
cow ... ALPI(280993)
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一般說明
碱性磷酸酶是一种磷酸单酯酶，可催化核酸的末端磷酸盐裂解。游离 5′-OH 可用多核苷酸激酶和 γ32P-ATP 磷酸化，以产生 γ32P 末端标记核酸。通过碱性磷酸酶去磷酸化可以防止线性化的克隆载体再环化。
應用
通常用于在分子克隆反应过程中从核酸中去除5'-末端磷酸盐。
成分
碱性磷酸酶以50% (w/v) 甘油溶液的形式提供，溶液中还含有 10 mM Tris-HCl (pH 8.2)、50 mM KCl、1 mM MgCl 2 和 0.1 mM ZnCl 2 。还包括 10X CIP 缓冲液（产品编号 C3225）。
單位定義
一个单位将在37℃ 下每分钟水解 1.0μ摩尔对 -硝基苯磷酸盐。
其他說明
5 mM EDTA (pH 8) 中的碱性磷酸酶将在 75℃ 下不可逆地加热灭活 10 分。
抑制劑
相關產品
產品號碼
描述
訂價
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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條款
CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.
CIP 用於去除 DNA、RNA 以及核糖核苷三磷酸鹽和脫氧核糖核苷三磷酸鹽上的 5'- 磷酸基團。詳細說明如何使 DNA 去磷酸化。
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)
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