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應用
丙酮酸氧化酶(PoxB)可将丙酮酸直接转化为乙酸盐和CO2。它可用于研究丙酮酸的代谢。用于研究细菌的有氧代谢,例如 植物乳杆菌和 肺炎链球菌。
来自气球菌的丙酮酸氧化酶已被用于丙酮酸的基于Amplex Red的荧光分析中。 它适用于制备用于测定硫胺素(维生素B1)的生物传感器, 以及作为多酶生物催化级联反应恒定部分的组成部分,从而用于确定创伤性脑损伤(TBI)和软组织损伤(STI)的生物标志物。
生化/生理作用
丙酮酸氧化酶可将丙酮酸氧化形成乙酸盐、过氧化氢和二氧化碳。 丙酮酸氧化酶需要黄素腺嘌呤二核苷酸(FAD)、焦磷酸硫胺素(TPP)和镁作为其催化活性的辅助因子。硫胺素可激活丙酮酸氧化酶活性。
丙酮酸氧化酶由四个具有相同分子量的亚基组成。PoxB可与某些醛反应,并且磷酸盐可以被砷酸盐代替。氧气以及几种人造化合物都可以充当电子受体。丙酮酸氧化酶可被磷脂以及单体和胶束两亲物活化。
單位定義
在37°C、pH 6.7 条件下,在丙酮酸和磷酸盐转化为乙酰磷酸盐和 CO2 的过程中,一单位丙酮酸氧化酶每分钟可产生 1.0 μmole H2O2。
外觀
含有缓冲盐和糖的冻干粉
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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