推薦產品
形狀
buffered aqueous solution
比活性
10-40 units/mg protein (biuret)
運輸包裝
dry ice
儲存溫度
−20°C
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一般說明
酒精氧化酶(AOX)是一种均八聚物,分子量为600-kDa。它具有八个黄素腺嘌呤二核苷酸(FAD)辅因子。它属于葡萄糖-甲醇-胆碱(GMC)氧化还原酶家族。
應用
毕赤酵母来源酒精氧化酶溶液用于棉质果胶甲基酯酶(PME)的酶测定中。它也可以可视化的生物传感器形式固定在聚苯胺(PANI)膜上,以试纸的形式用于测定发酵饮料样品中的乙醇。
乙醇氧化酶可用于研究蛋白向过氧化物酶体的转位。产品 A2404 来自 毕赤酵母 。已用于 Hv-p68 cDNA 克隆的细菌表达和免疫学验证 。
生化/生理作用
醇氧化酶催化短链初级脂肪醇氧化成各自的醛。对甲醇的亲和力最高。醇氧化酶参与甲醇的代谢,这对利用甲醇作为唯一能源的酵母菌来说很重要。乙醇氧化酶主要存在于过氧化物酶体,但也可能存在于细胞质中。
單位定義
一个单位将在 pH 7.5 下在 25°C 下每分钟将 1.0μmole 甲醇氧化成甲醛。
外觀
30% 蔗糖溶液,含 0.1 M 磷酸盐缓冲液 (pH 8.0)
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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條款
To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.
相關內容
This procedure may be used for all Alcohol Oxidase products. The continuous spectrophotometric rate determination (A405, Light path = 1 cm) is based on the following reactions.
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