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重要文件

P3288

Sigma-Aldrich

磷酸盐缓冲液，25 °C，pH 7.2

for hematology and histology staining techniques

同義詞:

磷酸缓冲液

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About This Item

分類程式碼代碼:
12352107
NACRES:
NA.47
暫時無法取得訂價和供貨情況

形狀

powder

顏色

white

pH值

7.10-7.30

應用

hematology
histology

儲存溫度

room temp

相關類別

一般說明

磷酸盐缓冲液是手术灌洗液的最常用组分，用于保存活组织。它也用于免疫化学染色流程。磷酸盐缓冲液是光学和电子显微镜的重要固定剂。其缓冲能力对pH敏感，最好在生理pH（7.2-7.4）下使用。[1]

應用

适合用作各种Romanowsky型染色流程的缓冲液，包括Wright Stain、Wright Giemsa、Giemsa、May Grunwald、Jenner和Leishman。在25℃下pH 7.2的磷酸盐缓冲液已被用于收集猪卵巢的卵母细胞。[2]

重構

每瓶可制备3.8 L缓冲液，8.3 mM。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

Lot/Batch Number
0000418704
0000418704
0000376999
0000325234
0000376999

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Low concentrations of MEM vitamins during in vitro maturation of porcine oocytes improves subsequent parthenogenetic development
Naruse K, et al.
Theriogenology, 67(2), 407-412 (2007)
Biological Electron Microscopy: Theory, Techniques, and Troubleshooting (2011)
Thaise L Teixeira et al.
Microbial pathogenesis, 135, 103618-103618 (2019-07-17)
P21 is a protein secreted by Trypanosoma cruzi (T. cruzi). Previous studies have shown a spectrum of biological activities performed by P21 such as induction of phagocytosis, leukocyte chemotaxis and inhibition of angiogenesis. However, the activity of P21 in T.

相關內容

血液學

了解血液、造血器官和血液疾病的臨床研究，包括治療、預防以及血液學測試中使用的染色劑和染料。

Hematology

Learn about the clinical study of blood, blood-forming organs, and blood diseases including the treatment, prevention, and stains and dyes used in hematology testing.

Questions

1–4 of 4 Questions  

  1. could you provide me with the Wright-Giemsa protocol referenced in the question above about how to solubilize Product P3288, Phosphate Buffer pH 7.2. We need official instructions for use and the product did not come with it.

    1 answer

    1. If this product is ordered separately, no instructions are provided. Instructions for preparing P3288 are found in the WG Instructions for Use that ship with each bottle of WG16, WG32, WG80, or WG128. Below is the information from the Reagent section of the WG procedure Instructions For Use

      The phosphate buffer should be prepared by diluting 1 vial of buffer to 1 gallon or 3.8 liters of deionized water. Mix well to dissolve. Discard the working phosphate solution if turbidity or visible bacterial growth is present. Store the working phosphate buffer solution at 2-8°C

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  2. Once made how long is the solution good for?

    1 answer

    1. The long term stability of this product in solution has not been evaluated. Upon reconstitution, the solution should be observed for any particulate matter or turbidity which would indicate possible microbial growth. However, similar prepared solutions such as P3619, a 1M Phosphate Buffer, are not assigned an expiration date. These products are quite stable and offer a warranty of 1 year from the date of shipment.

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  3. How to solubilize Product P3288, Phosphate Buffer pH 7.2?

    1 answer

    1. The instructions for reconstitution of Product P3288, Phosphate buffer pH 7.2 at 25 °C are indicated on the Wright-Giemsa protocol (see attached packaging insert).Briefly, Product P3288, Phosphate buffer pH 7.2 should be prepared by diluting 1 vial of buffer to 1 gallon or 3.8 liters of deionized water. Mix well to dissolve. Store Phosphate Buffer at room temperature (18-26°C). Store working phosphate buffer solution at 2-8°C. Discard the working phosphate solution if turbidity or visible bacterial growth is present.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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