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一般說明
1步法: 同一管中的eAMV™ RT逆转录酶和AccuTaq LA DNA聚合酶相继发挥作用,首先生成cDNA,紧接着进行PCR扩增反应。 用于灵敏、快速的RNA分析。
2步法: 每次反应都经过单独优化,可在高保真前提下获得更高产率。适合需要更多扩增的实验,或高产率比便利性更加重要的实验。
特點和優勢
- 该反转录酶可制备更长的转录本,生成长达14.1kb的cDNA。
- 低丰度mRNA检测灵敏度更高。eAMV™ RT能够成功转录其他反转录酶无法检测到的微量RNA。
- 针对复杂的二级结构的RNA,拥有超强的转录能力。
- JumpStart AccuTaq LA DNA聚合酶有利于提高灵敏度、特异性与得率。
其他說明
法律資訊
危險聲明
防範說明
危險分類
Aquatic Chronic 3
儲存類別代碼
10 - Combustible liquids
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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相關內容
The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.
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