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N3520

Sigma-Aldrich

营养混合物 F-12 Ham

Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

同義詞:

Ham's F-12

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

形狀

powder

技術

cell culture | mammalian: suitable

成分

NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

营养混合物Ham#8242;s F-12由Ham开发,其含有较高水平的氨基酸、维生素和微量元素。其将腐胺和亚油酸加入配方中。该营养培养基最初设计用于中国仓鼠卵巢和肺细胞的无血清生长。当与全血或透析血清一起使用或与激素和转铁蛋白组合使用时,它可广泛用于哺乳动物和杂交瘤细胞的生长。

應用

营养混合物F-12 Ham可用于以下应用:
  • HeLa(人子宫颈腺癌细胞)细胞在补充有10%热灭活胎牛血清的该培养基(营养混合物F-12 Ham)进行中生长
  • 在维持和培养期间用于不同细胞的细胞培养(在营养混合物F-12 HAM培养基与其他组分中,A549细胞系保持为单层)
  • 用作Cowdria ruminantium繁殖的培养基
  • 用于制备初级混合胶质细胞培养物

Kaighn#8242;s 改良的Ham#8242;s F-12和Coon#8242;s F-12增加了氨基酸和丙酮酸,以及改性盐(Konigsbergs)的浓度。该培养基被设计用于支持分化的大鼠和鸡细胞以及原代人肝细胞的生长。

數量

配方中每升培养基含有11.1克的粉末。

重構

并补充2.5 g/L碳酸氢钠。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

使用低溫保存的 NK 效應細胞進行 ADCC 分析的逐步方案,包括提示和技巧、分析開發和資料分析方法。

粉狀介質和鹽混合物具有極強的吸濕性,因此應防止大氣中的濕氣進入。

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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