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MAK194

Sigma-Aldrich

脂质(油红O)染色试剂盒

Sufficient for 2 96-well plates

同義詞:

油红O脂染色试剂盒

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.25
暫時無法取得訂價和供貨情況

相關疾病

endocrinological disorders, diabetes; obesity; cardiovascular diseases

儲存溫度

room temp

一般說明

脂滴(LD)是动态的、普遍存在的脂质储存细胞器,主要存在于脂肪细胞中。存储在LD中的甘油三酯、中性脂质和胆固醇酯是最大的能量来源。脂肪细胞中LD过量可导致肥胖和肥胖相关病症,如血脂异常和糖尿病。

應用

脂质(油红O)染色试剂盒用于对样品中的脂质[1]和脂肪细胞[2]进行染色。

適合性

此试剂盒适于染色中性脂质,可用于两块96孔板、两块6孔板或者四个100mm培养皿。

原則

脂质(油红O)染色试剂盒适用于对培养细胞的中性脂质进行选择性染色和检测。试剂盒中包含的苏木精可对细胞核进行染色。

僅套裝組件

產品號碼
描述

  • PBS

  • Formalin (10%)

  • Oil Red O

  • Hematoxylin

象形圖

Skull and crossbonesHealth hazard

訊號詞

Danger

危險分類

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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分析證明 (COA)

Lot/Batch Number

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Kefeng Yang et al.
Xenotransplantation, 24(5) (2017-07-19)
Survival and longevity of xenotransplants depend on immune function and ability to integrate energy metabolism between cells from different species. However, mechanisms for interspecies cross talk in energy metabolism are not well understood. White adipose tissue stores energy and is
Yohei Nomura et al.
Frontiers in physiology, 12, 675724-675724 (2021-07-06)
We previously reported that histone deacetylase 6 (HDAC6) has an important role in endothelial cell (EC) function in vitro. However, whether HDAC6 plays a role in atherogenesis in vivo and the mechanism(s) that control HDAC6 activity/expression in response to atherogenic
CD36 mediates lipid accumulation in pancreatic beta cells under the duress of glucolipotoxic conditions: Novel roles of lysine deacetylases.
Khan S and Kowluru A
Biochemical and Biophysical Research Communications, 495(3), 2221-2226 (2018)
Aldehyde dehydrogenase 1 a1 regulates energy metabolism in adipocytes from different species.
Yang K, et al.
Xenotransplantation, 24(5), e12318-e12318 (2017)
Jonathan J Hernandez et al.
Journal of translational medicine, 19(1), 149-149 (2021-04-16)
Mesenchymal stem cells derived from human umbilical cord (hUC-MSCs) have immunomodulatory properties that are of interest to treat novel coronavirus disease 2019 (COVID-19). Leng et al. recently reported that hUC-MSCs derived from one donor negatively expressed Angiotensin-Converting Enzyme 2 (ACE2)

Questions

1–3 of 3 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. What are the methods for removing red stains from glassware from kit MAK194?

    1 answer
    1. For cleaning glassware from kit MAK194, it is suggested to rinse the glassware with ethanol and then scrub it with warm tap water and soap to remove the red staining caused by the Oil Red O solution. Following these steps with a standard wash in a lab dishwasher should yield optimal results. It's important to note that the longer the solution remains in the glassware, the more difficult it becomes to remove. Therefore, it is recommended to prepare special staining solutions in one-use plastic bottles to avoid this issue.

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