The high background issue could be due to the use of antifade, which is not recommended. Instead, it's suggested to use PBS as the mounting medium and nail polish as the coverslip sealant. Additionally, using other mounting media such as EverBrite™ or FluoroShield™ may impact staining patterns, so it's important to consider the recommended mounting methods to avoid issues with signal intensity and staining patterns.
推薦產品
技術
cell based assay: suitable
檢測方法
fluorometric
運輸包裝
ambient
一般說明
BioTracker脂质染料可快速染色活细胞或固定细胞内的脂滴,过程中无需洗涤,对其他细胞膜、细胞器的背景染色极低。染色后还可进行细胞固定和透化。
BioTracker 488绿色脂质染料的激发波长约为430 nm,在405 nm或488 nm处的激发效果一样好。染料将细胞中的脂滴染成亮绿色荧光,可以用FITC通道检测。
光谱特性
激发: 427nm
发射:585nm
應用
活细胞染料
细胞成像
品質
激发: 427nm
发射:585nm
外觀
儲存和穩定性
注意:打开小瓶前,短暂离心小瓶以收集小瓶底部的内容物。
免責聲明
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
188.6 °F - (refers to pure substance)
閃點(°C)
87 °C - (refers to pure substance)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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Are there any solutions for the high background issue experienced when using the product with heart tissue cells?
1 answer-
Helpful?
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Is BioTracker 488 Green Lipid Dye (SCT144) toxic for the cells? Can they be monitored for extra time after staining? How long does the stain last in this case?
1 answer-
No cytotoxicity of this dye has been observed with incubation periods of up to 24 hours. Cytotoxicity beyond the 24-hour incubation period has not been studied. Suitability for extended incubation times would have to be determined by the end user.
Helpful?
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