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MAK143

Sigma-Aldrich

细胞内ROS检测试剂盒（绿色荧光）

sufficient for 200 fluorometric tests (green)

同義詞:

荧光ROS检测试剂盒

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.25
暫時無法取得訂價和供貨情況

用途

sufficient for 200 fluorometric tests (green)

檢測方法

fluorometric

相關疾病

cancer; neurological disorders

儲存溫度

−20°C

相關類別

一般說明

活性氧(ROS)的产生是由于有氧呼吸过程中氧的减少和细胞内各种酶系统的作用。在生理水平上，ROS有助于细胞信号传导和宿主防御。超过了生物系统的解毒能力的活性氧产生，导致氧化应激和细胞损伤。细胞的主要损伤是由ROS诱导的大分子的改变，如膜脂、必需蛋白和DNA中多不饱和脂肪酸等。活性氧与阿尔茨海默′s疾病、帕金森病、癌症和衰老等疾病状态有关。

應用

荧光细胞内ROS试剂盒已可用于活性氧(ROS)的生成检测。[1]

適合性

本试剂盒适于通过酶标仪或荧光显微镜检测细胞内的ROS。

原則

荧光细胞内ROS检测试剂盒提供了一种灵敏的、一步荧光检测方法，可在1小时内检测活细胞内的ROS(特别是超氧化物和羟基自由基)。ROS与定位于细胞质的荧光传感器发生反应，产生荧光产物(λex = 490/λem = 520 nm)产物，与ROS含量成正比。该试剂盒可以采用96孔或384孔多孔板形式，并使用荧光显微镜或荧光微孔板读板仪进行读取。

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
3760362
3760362
3510274
3510274
3181833

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Alberto Sola-Leyva et al.
Journal of materials chemistry. B, 8(34), 7667-7676 (2020-07-25)
Recent studies have shown the potential of magnetic hyperthermia in cancer treatments. However, the underlying mechanisms involved have not been yet fully described. In particular, the cell death related to magnetic hyperthermia observed in cultures incubated with low concentration of
Yushan Xia et al.
PLoS pathogens, 17(9), e1009909-e1009909 (2021-09-04)
The emergence and rapid spread of multi-drug resistant (MDR) bacteria pose a serious threat to the global healthcare. There is an urgent need for new antibacterial substances or new treatment strategies to deal with the infections by MDR bacterial pathogens
Almira Georgieva et al.
Antioxidants (Basel, Switzerland), 10(10) (2021-10-24)
The wastewater from the distillation of rose oils is discharged directly into the soil because it has a limited potential for future applications. The aim of the present study was to determine in vitro the chromatographic profile, redox-modulating capacity, and
Multi-functionality redefined with colloidal carotene carbon nanoparticles for synchronized chemical imaging, enriched cellular uptake and therapy.
Misra S K, et al.
Scientific Reports, 6, 29299-29299 (2016)
Maiara Voltarelli Providello et al.
Antimicrobial agents and chemotherapy, 62(9) (2018-07-11)
The acute phase of Chagas disease (CD) is characterized by high parasitic proliferation and intense inflammation, exacerbating the generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS). These reactive molecules are also increased by the metabolism of the
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Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Does the fluorescence produced by ROS resist PFA fixation? Is it detergent resistant?

    1 answer

    1. This kit is intended to be used on living cultured cells. It is not recommended for use on paraformaldehyde (PFA) fixed cells or tissue, or on cells or tissue that have been treated with a detergent or surfactant.

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  4. What is the mechanism for detecting ROS? Specifically, what component in the ROS Detection Reagent enables the detection of ROS upon their formation?

    1 answer

    1. The component A ROS green is a non-conjugated compound that is concealed with a ROS-cleavable blocking group. Upon the ROS reaction, the blocking group is cleaved by ROS to release a strongly fluorescent dye. The ROS production is proportional to the concentration of the fluorophore.

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  5. Is ROS Detection Reagent(Green) the same as 2′,7′-Dichlorofluorescin diacetate?

    1 answer

    1. The composition information for the ROS Detection Reagent, Green solution is considered prioprietary.

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