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MAK145

Sigma-Aldrich

细胞内ROS检测试剂盒(红色荧光)

sufficient for 200 fluorometric tests (red)

同義詞:

Intracellular ROS Fluorescence Assay

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.25

用途

sufficient for 200 fluorometric tests (red)

檢測方法

fluorometric

相關疾病

neurological disorders; cancer

儲存溫度

−20°C

一般說明

活性氧 (ROS) 是由于有氧呼吸过程中氧气减少和细胞内各种酶系统产生的。在生理水平,活性氧有助于细胞信号和宿主防御。ROS 生成增加,高于生物系统的解毒能力,导致氧化应激和细胞损伤。对细胞的主要损伤是由 ROS 诱导的膜脂、必需蛋白质和 DNA 中的多不饱和脂肪酸等大分子的改变所致。ROS 与阿尔茨海默病、帕金森病、癌症、衰老等疾病状态有关。

應用

荧光细胞内活性氧 (ROS) 试剂盒已用于检测活细胞中的细胞内 ROS。

適合性

本试剂盒适于通过酶标仪或荧光显微镜检测细胞内的ROS。

原則

试剂盒提供灵敏的一步荧光测定方法,不用孵育1小时即可检测活细胞的胞内 ROS(特别是超氧化物和羟基自由基)。ROS与细胞透过性传感器反应,生成的荧光产物(λex = 520/λem = 605 nm)与 ROS 量成比例。这一测定可用荧光微板读取仪,在96孔或384孔多孔板中进行。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Neurodegenerative diseases and oxidative stress.
Barnham K J, et al.
Nature Reviews. Drug Discovery, 3(3), 205-205 null
Sean A Misek et al.
Frontiers in oncology, 12, 766794-766794 (2022-04-22)
Single agent and combination therapy with BRAFV600E/K and MEK inhibitors have remarkable efficacy against melanoma tumors with activating BRAF mutations, but in most cases BRAF inhibitor (BRAFi) resistance eventually develops. One resistance mechanism is reactivation of the ERK pathway. However
Yara E Elakkad et al.
Breast cancer (Dove Medical Press), 13, 675-689 (2021-12-23)
Female breast cancer is the most prevalent cancer worldwide. Emerging evidence shows that simvastatin (SIM) has promising anticancer activities. However, the underlying mechanisms are not fully elucidated. Increasing reports imply statins can modulate ferroptosis through disrupting reactive oxygen species (ROS)
Jianyong Yu et al.
Experimental and therapeutic medicine, 22(3), 946-946 (2021-07-27)
Salinomycin is a polyether antiprotozoal antibiotic that is widely used as an animal food additive. Some antifungal, antiparasitic, antiviral and anti-inflammatory activities have been reported for salinomycin. Recently, the anti-cancer effect of salinomycin has been demonstrated in breast cancer; however
Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants.
Das K and Roychoudhury A
Frontiers in Endocrinology, 2, 53-53 (2014)

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