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Merck
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重要文件

287810

Sigma-Aldrich

2′,7′-二氯荧光素二醋酸盐

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.

同義詞:

2′,7′-二氯荧光素二醋酸盐, H₂DCFDA, 2ʹ,7ʹ-二氯二氢荧光素二乙酸酯

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About This Item

經驗公式(希爾表示法):
C24H16Cl2O7
CAS號碼:
分子量::
487.29
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.25
暫時無法取得訂價和供貨情況

品質等級

化驗

≥94% (HPLC)

形狀

crystalline solid

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
protect from light

溶解度

DMSO: 5 mg/mL

運輸包裝

ambient

儲存溫度

−20°C

SMILES 字串

Clc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)Cl)C2c4c(cccc4)C(=O)O

InChI

1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChI 密鑰

PXEZTIWVRVSYOK-UHFFFAOYSA-N

一般說明

细胞可透过性荧光探针,可用于检测中性粒细胞和巨噬细胞中的活性氧(ROS)和一氧化氮(NO),测定毒理学现象中的整体氧化应激程度。

生化/生理作用

主要目的
检测活性氧(ROS)和一氧化氮(•NO)
产品不与ATP竞争。
可逆:否
细胞渗透性:是

包裝

用惰性气体包装

警告

毒性:标准处理(A)

重構

溶解后,等分并冷冻保存(-20°C)。储备溶液在-20°C下可稳定保存至多3个月。

其他說明

Crow, J.P. 1997.Nitric Oxide 1, 145.
Ripple, M.O., et al. 1997.J. Natl.Cancer Inst.89, 40.
Caro, A., and Puntarulo, S. 1996.Biochim.Biophys.Acta1291, 245.
Perez-Garcia, F., et al. 1996.Life Sci. 59, 2033.
Ramakrishnan, N., et al. 1996.生物化学。Pharmacol.51, 1443.
Ferrer, A.S., et al. 1990.Anal.生物化学。187, 129.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


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Questions

  1. Hi! Can you please share the protocol of 287810 (2′,7′-Dichlorofluorescin Diacetate) Best,

    1 answer
    1. This product has not been tested for any specific application, so a specific protocol is not available. However, this material has been used successfully by a number of researchers.
      Please see the link below to review publications that may be helpful:
      https://www.sigmaaldrich.com/search/287810?focus=papers&page=1&perpage=30&sort=relevance&term=287810&type=citation_search

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