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MAK071

Sigma-Aldrich

丙酮酸检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

同義詞:

丙酮酸检测测定试剂盒

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84
暫時無法取得訂價和供貨情況

用途

sufficient for 100 colorimetric or fluorometric tests

檢測方法

colorimetric
fluorometric

相關疾病

gastrointestinal diseases; cancer

儲存溫度

−20°C

一般說明

丙酮酸是代谢中的中心分子,其通过多种代谢途径进行使用和合成,其中包括糖酵解和丙氨酸的氨基转移作用。丙酮酸可在柠檬酸循环中进一步氧化,在糖异生期间转化为碳水化合物,或还原为乳酸。高水平的丙酮酸与肝脏疾病和遗传性疾病有关。丙酮酸也被用于刺激代谢,从而导致体重减轻。

適合性

适于测定血液、组织、培养细胞等多种生物样品中的丙酮酸浓度。

原則

由偶联酶测定法测定丙酮酸浓度,得到的比色(570nm)/荧光(λex = 535/λem =587 nm)产物与存在丙酮酸成比例。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids


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Abstracts of the XVIth International Conference on Biological Inorganic Chemistry, July 22-26, 2013, Grenoble, France.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 19 Suppl 1, 1-696 (2014-03-13)
Lauren K Wareham et al.
Antioxidants & redox signaling, 24(17), 1013-1028 (2016-02-26)
Carbon monoxide is a respiratory poison and gaseous signaling molecule. Although CO-releasing molecules (CORMs) deliver CO with temporal and spatial specificity in mammals, and are proven antimicrobial agents, we do not understand the modes of CO toxicity. Our aim was
Changes in muscle cell metabolism and mechanotransduction are associated with myopathic phenotype in a mouse model of collagen VI deficiency.
De Palma S, et al.
PLoS ONE, 8(2), e56716-e56716 (2013)
Sara De Palma et al.
PloS one, 8(2), e56716-e56716 (2013-02-26)
This study identifies metabolic and protein phenotypic alterations in gastrocnemius, tibialis anterior and diaphragm muscles of Col6a1(-/-) mice, a model of human collagen VI myopathies. All three muscles of Col6a1(-/-) mice show some common changes in proteins involved in metabolism
Claire G Jeong et al.
Acta biomaterialia, 10(8), 3421-3430 (2014-05-27)
Hyaluronic acid (HA)-poly(ethylene glycol) (PEG) composite hydrogels have been widely studied for both cell delivery and soft tissue regeneration applications. A very broad range of physical and biological properties have been engineered into HA-PEG hydrogels that may differentially affect cellular

文章

沃伯格效應會增強腫瘤細胞中葡萄糖到乳酸的轉換,而不受氧氣水平的影響;自 1924 年起就開始影響癌症新陳代謝。

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. Can I warm my pyruvate Assay buffer to 37°C to dissolve the precipitated flakes at the bottom, or will that disrupt the assay?

    1 answer
    1. All components should be thawed at room temperature. If the components are fully thawed at room temperature and there is still precipitate in the Assay buffer, follow this step: It is advised that the buffer can be kept at 37°C for a short time to see if it helps. This brief exposure to 37°C should not negatively affect the assay; in fact, many researchers keep the buffer bottle at this temperature in a water bath while thawing it.

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