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重要文件

L5667

Sigma-Aldrich

LB Agar Ampicillin-100, Plates

pre-poured LB agar plates with 100 μg/ml ampicillin

同義詞:

ampicillin agar plates

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About This Item

分類程式碼代碼:
41121800
暫時無法取得訂價和供貨情況

等級

Molecular Biology
for molecular biology

無菌

sterile; autoclaved

形狀

plates (10 cm)
plates (10cm, 20-25ml each)

存貨情形

available only in USA

容量

20-25 mL

儲存溫度

2-8°C

適合性

selective for Escherichia coli
selective for coliforms

一般說明

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

應用

LB Agar Ampicillin-100, Plates has been used to plate and incubate the mixture of thawed bacteria and plasmid solution for vimentin expression.[1] It has also been used in the generation of the Trypanosoma brucei RNAi library.[2]

特點和優勢

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

成分

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
100μg/ml ampicillin

其他說明

The plates must be used within three months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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Linfeng Xu et al.
Nature communications, 12(1), 6803-6803 (2021-11-25)
Enzymes are represented across a vast space of protein sequences and structural forms and have activities that far exceed the best chemical catalysts; however, engineering them to have novel or enhanced activity is limited by technologies for sensing product formation.
Genome-scale RNAi screens for high-throughput phenotyping in bloodstream-form African trypanosomes
Glover L, et al.
Nature Protocols, 10(1), 106-106 (2015)
Daniel Del Valle Morales et al.
Bio-protocol, 10(20), e3791-e3791 (2021-03-05)
The 5' cap is a ubiquitous feature of eukaryotic mRNAs. It is added in the nucleus onto newly synthesized pre-mRNA, and in the cytoplasm onto mRNAs after decapping or endonuclease cleavage. Cytoplasmic recapping can occur after loss of the cap
Viscoelastic properties of vimentin originate from nonequilibrium conformational changes
Block J, et al.
Science Advances, 4(6) (2018)
Viscoelastic properties of vimentin originate from nonequilibrium conformational changes
Block J, et al.
Science advances, 4(6) (2018)

文章

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

基因工程可以大規模地表達和分離用於研究目的的重組蛋白質。

Questions

1–2 of 2 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. Why do the LB-agar plates with antibiotics have such a short expiration date?

    1 answer
    1. The antibiotics in the LB-agar plates are not stable.  The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate.  Because of this, we recommend the plates are used as soon as possible once they are received.  For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).

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