The antibiotics in the LB-agar plates are not stable. The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate. Because of this, we recommend the plates are used as soon as possible once they are received. For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).
推薦產品
等級
Molecular Biology
for molecular biology
無菌
sterile; autoclaved
形狀
plates (10 cm)
plates (10cm, 20-25ml each)
存貨情形
available only in USA
容量
20-25 mL
儲存溫度
2-8°C
適合性
selective for Escherichia coli
selective for coliforms
一般說明
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
應用
Suitable for selective cultivation of E. coli strains that contain plasmids conferring ampicillin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins.
特點和優勢
Miller′s LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation plus antibiotic
成分
15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml ampicillin
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml ampicillin
其他說明
The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.
相關產品
產品號碼
描述
訂價
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
文章
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
基因工程可以大規模地表達和分離用於研究目的的重組蛋白質。
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Why do the LB-agar plates with antibiotics have such a short expiration date?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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