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L5418

Sigma-Aldrich

脂多糖 来源于大肠杆菌 055:B5

Ready Made solution, 1 mg/mL

同義詞:

LPS

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About This Item

MDL號碼:
分類程式碼代碼:
12352201
NACRES:
NA.25

生物源

Escherichia coli (O55:B5)

品質等級

形狀

aqueous solution

濃度

1 mg/mL

顏色

white to faint yellow

溶解度

water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

運輸包裝

wet ice

儲存溫度

2-8°C

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一般說明

本品为 0.2 μm 过滤水溶液,含 1 mg/mL 来自大肠杆菌血清型 O55:B5 的脂多糖 (LPS)。来源菌株为 CDC 1644-70。这种 LPS 血清型已被用于感染性休克和心肌炎的研究。

應用

脂多糖(LPS)是革兰氏阴性细菌细胞壁的特征组分。脂多糖及其脂质 A 部分通过 Toll 样受体 4(TLR4)刺激先天免疫系统的细胞,Toll 样受体 4是 Toll 样受体蛋白家族的成员,其识别常见的病原体相关分子模式(PAMP)。

生化/生理作用

脂多糖位于膜的外层,在非包膜菌株中,暴露在细胞表面。它们有助于外膜的完整性,保护细胞免受胆盐和亲脂性抗生素的作用。

準備報告

用于制备溶液的 LPS 经过苯酚提取和凝胶过滤层析纯化。通过苯酚提取制备的脂多糖含有高达 60% 的 RNA 和少于 1% 的蛋白质。随后通过凝胶过滤色谱法进行纯化,可去除苯酚提取的 LPS 中存在的大部分蛋白质,但留下的产物仍含有 10-20% 的核酸。采用离子交换色谱法进一步纯化,得到含 < 1% 蛋白质和 < 1%RNA 的 LPS 产品。

其他說明

为了全面了解我们针对客户研究提供的各种脂多糖产品,建议您访问我们的碳水化合物分类页面。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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文章

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

相關內容

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

脂多醣 (LPS) 是革蘭氏陰性細菌的主要成分,對微生物研究非常重要。

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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