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重要文件

L4516

Sigma-Aldrich

脂多糖 来源于大肠杆菌 0127:B8

BioXtra, suitable for cell culture, γ-irradiated

同義詞:

LPS

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352211
NACRES:
NA.75
暫時無法取得訂價和供貨情況

生物源

Escherichia coli (O127:B8)

品質等級

無菌

γ-irradiated

產品線

BioXtra

形狀

lyophilized powder

純化經由

gel-filtration chromatography

技術

cell culture | mammalian: suitable

雜質

<1% Protein (Lowry)

溶解度

H2O: 5 mg/mL, slightly hazy

儲存溫度

2-8°C

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應用

来自Escherichia coli 0127:B8脂多糖用于:
  • 研究LPS诱导小鼠巨噬细胞存活[1]
  • 诱导牛PBMC分泌细胞因子[2]
  • 研究LPS诱导促炎环境高易感巨噬细胞细胞凋亡。[1]
  • 进行BV2和BV2-LC3细胞自吞噬实验。[3]
  • 激活人单核细胞,巨噬细胞和小鼠小胶质细胞[4][5][6][7]
  • 诱导293T细胞穿透素-3[8]
脂多糖(LPS)是革兰氏阴性菌细胞壁的特征组分。LPS及其脂质A部分可通过能够识别常见的病原体相关分子模式(PAMP)的Toll样受体蛋白家族成员Toll样受体4(TLR4)刺激先天性免疫系统细胞。

生化/生理作用

脂多糖(LPS)是革兰氏阴性菌细胞壁的特征组分。LPS和它的脂质A单体通过Toll样受体4(TLR4)激活天然免疫系统细胞。作为免疫原抗原,它增强了对于可溶性抗原的免疫反应。[9][10]

重構

脂多糖以经γ-射线处理的冻干粉形式提供。如需重悬,可将1 ml无菌的平衡盐溶液或组织培养基加入至小瓶(1 mg)中并轻轻晃动直至粉末溶解。重悬后的产品可采用无菌的平衡盐溶液或组织培养基进一步被稀释至所需的工作浓度。

其他說明

为了全面了解我们针对客户研究提供的各种脂多糖产品,建议您访问我们的碳水化合物分类页面。

象形圖

Skull and crossbones

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 2 Oral

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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A Charlotte M T de Wolf et al.
PloS one, 12(6), e0179942-e0179942 (2017-06-29)
Regulatory T cells (Treg) function in the prevention of excessive inflammation and maintenance of immunological homeostasis. However, these cells may also interfere with resolution of infections or with immune reactions following vaccination. Effects of Treg on vaccine responses are nowadays
Hye-Eun Han et al.
Biomolecules & therapeutics, 21(1), 21-28 (2013-09-07)
Microglia play a role in maintaining and resolving brain tissue homeostasis. In pathological conditions, microglia release pro-inflammatory cytokines and cytotoxic factors, which aggravate the progression of neurodegenerative diseases. Autophagy pathway might be involved in the production of pro-inflammatory cytokines and
Li Wei et al.
Journal of neuroinflammation, 14(1), 198-198 (2017-10-05)
Several G-protein-coupled receptors (GPCRs) have been shown to be important signaling mediators between neurons and glia. In our previous screening for identification of nerve injury-associated GPCRs, G-protein-coupled receptor 84 (GPR84) mRNA showed the highest up-regulation by microglia after nerve injury.
Hua Liu et al.
International journal of clinical and experimental pathology, 6(4), 660-668 (2013-04-11)
The aim of the present study was to explore the role of receptor tyrosine kinases (RTKs) in the regulation of expression of PTX-3, a protector in atherosclerosis. Human monocytic U937 cells were infected with a shRNA lentiviral vector library targeting
Srinivas Akula et al.
Cells, 9(9) (2020-09-23)
Bone marrow-derived mast cells (BMMCs) are often used as a model system for studies of the role of MCs in health and disease. These cells are relatively easy to obtain from total bone marrow cells by culturing under the influence

文章

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Questions

1–4 of 4 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Once reconstituted what is the stability at -20C for the LPS product L4516-1M?

    1 answer
    1. Frozen aliquots can be stored up to 2 years. For more information, please see the link below:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

      Helpful?

  4. Can LPS bind to plastics (ex. 1.5 mL microtube) even when LPS solution is frozen stored at -20℃? I store LPS solution at 0.05mg/mL in -20℃ now.

    1 answer
    1. LSP is less likely to stick to plastics than glass, however very low concentrations (<0.1 mg/mL in water), LPS tends to stick to containers made
      of certain types of glass or plastic. To minimize, prepare stock solutions of at least 1 mg/mL. If the LPS concentration is >1 mg/mL, adsorption to the sides of the vial is
      negligible. For solutions stored in glass, vortexing for 30 minutes following thaw is recommended to redissolve. This may be useful for dilute solutions in plastic, as well. Surfaces (glass and plastics) may be salinized to reduce adherence.

      See Sigmacote, SL2:
      https://www.sigmaaldrich.com/product/sigma/sl2

      For additional information, see the link below to review the Lipopolysaccharides product information page:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

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