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Merck
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重要文件

L4524

Sigma-Aldrich

脂多糖 来源于大肠杆菌 055:B5

purified by ion-exchange chromatography, TLR ligand tested

同義詞:

LPS

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352201
NACRES:
NA.25
暫時無法取得訂價和供貨情況

生物源

Escherichia coli (O55:B5)

品質等級

形狀

lyophilized powder

純化經由

ion-exchange chromatography

雜質

<1% Protein
<1% RNA

顏色

white to yellow cast

溶解度

water: soluble

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

本产品是从大肠杆菌血清型 O55:B5 中提取而来,并通过离子交换进行了纯化。源菌株是 CDC 1644-70。LPS O55:B5 已用于刺激人腹膜巨噬细胞,以 1 ng/mL 的浓度,并以 1-100 ng/mL 的浓度刺激马腹膜巨噬细胞。

應用

脂多糖(LPS)是革兰氏阴性细菌细胞壁的特征组分。脂多糖及其脂质 A 部分通过 Toll 样受体 4(TLR4)刺激先天免疫系统的细胞,Toll 样受体 4是 Toll 样受体蛋白家族的成员,其识别常见的病原体相关分子模式(PAMP)。

生化/生理作用

脂多糖(LPS)位于膜的外层,并且在非包膜菌株中暴露在细胞表面上。它们有助于外膜的完整性,并保护细胞免受胆汁盐和亲脂性抗生素的影响。

準備報告

产物可溶于水(5mg/ml)或细胞培养基(1mg/ml),产生模糊的淡黄色溶液。在涡旋,并升温至 70-80 oC 后,在含水盐水中得到更浓缩但仍然浑浊的溶液(20 mg/ml)。脂多糖是在每种溶剂中形成胶束的分子。在水和磷酸盐缓冲盐水中观察到浑浊的溶液。有机溶剂不能提供更澄清的溶液。甲醇产生具有漂浮物的浑浊悬浮液,而水产生均匀浑浊溶液。

其他說明

为了全面了解我们针对客户研究提供的各种脂多糖产品,建议您访问我们的碳水化合物分类页面。

象形圖

Skull and crossbones

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 2 Oral

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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文章

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

相關內容

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

脂多醣 (LPS) 是革蘭氏陰性細菌的主要成分,對微生物研究非常重要。

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