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重要文件

L3768

Sigma-Aldrich

溶壁酶 来源于曲霉

powder

同義詞:

溶壁酶

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.54
暫時無法取得訂價和供貨情況

生物源

Aspergillus niger

形狀

powder

技術

cell based assay: suitable

顏色

brown

適合性

suitable for cell lysis

應用

cell analysis

儲存溫度

2-8°C

一般說明

Lysing enzyme(溶壁酶)也可称为裂解酶或细胞溶解酶。

應用

曲霉裂解酶已用于消化细胞壁制备脉冲场凝胶块。
该裂解酶(来自曲霉)用于:

  • 粟酒裂殖酵母(Schizosaccharomyces pombe)细胞壁消化[1]
  • 哈茨木霉(Trichoderma harzianum)菌丝体细胞壁裂解[2]

生化/生理作用

溶壁酶是可裂解微生物细胞壁的一类酶。在细胞裂解、组织解离和蛋白提取等多种生物过程中发挥重要作用。溶壁酶常用于研究、生物技术和临床应用。尤其适合用于DNA提取,可通过破裂细胞壁帮助释放细胞遗传物质,方便高效分离和纯化DNA用于下游分析。纤维素酶是能将植物细胞主要成分纤维素分解为更简单糖类物质的一类酶。这些糖类可作为原料生产生物燃料、生物基化学品、塑料和各种其他物质。果胶酶是分解微生物和高等植物中果胶物质的一类酶。可降解由植物和微生物合成的含果胶化合物。

品質

含有果胶酶和纤维素酶活性。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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存取文件庫

Tara L Mastro et al.
Journal of cell science, 133(10) (2020-04-23)
Translesion synthesis polymerases (TLSPs) are non-essential error-prone enzymes that ensure cell survival by facilitating DNA replication in the presence of DNA damage. In addition to their role in bypassing lesions, TLSPs have been implicated in meiotic double-strand break repair in
Pectinase from Microorganisms and Its Industrial Applications
Haile S and Ayele A
TheScientificWorldJournal (2022)
Translesion synthesis polymerases contribute to meiotic chromosome segregation and cohesin dynamics in Schizosaccharomycespombe
Mastro TL, et al.
Journal of Cell Science (2020)
Unleashing the Promise of Biotechnology to Help Heal, Fuel, and Feed the World
Greenwood JC
Journal of biological and chemical chronicles (2014)
New strains obtained after UV treatment and protoplast fusion of native Trichoderma harzianum: their biocontrol activity on Pyrenochaeta lycopersici
Besoain X A, et al.
Electronic journal of Biotechnology, 10(4), 604-617 (2007)

Questions

  1. What is the working condition and concentration of this enzyme, especially for filamentous fungi cell wall digestion

    1 answer
    1. The working conditions and concentration of this enzyme is not tested. This process will have to be validated by the end-user It may be helpful to view the peer-reviewed articles:

      https://link.springer.com/article/10.1007/BF00437577
      https://www.sciencedirect.com/science/article/pii/S1087184505001052
      https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2958.2005.04827.x

      Helpful?

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