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L3759

Sigma-Aldrich

来自西非单叶豆加纳籽)的凝集素

biotin conjugate, lyophilized powder

同義詞:

西非单叶豆 凝集素, BS-I

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

共軛

biotin conjugate

形狀

lyophilized powder

效力

<250 μg per mL agglutination activity (using human blood group A erythrocytes.)

成份

Protein, ~85% E1%/280

標籤範圍

~5 mol biotin per mol protein

儲存溫度

2-8°C

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應用

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used:
  • for highlighting murine endothelial cells
  • to identify fetal capillaries in endothelial immunohistochemical staining, conjugated with biotin
  • to detect tumor total vessels

生化/生理作用

BS-I对末端α-D-半乳糖残基具有主要亲和力,对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。

其他說明

BS-I是一种四聚体凝集素,由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素:BSI-B4、BSI-AB3、BSI-A2B2、BSI-A3B、以及BSI-A4。BSI-B4 是血型B特异的,其对末端α-D-半乳糖残基具有独特的亲和力,而BSI-A4 具有血型A特异性,其对末端N-乙酰基-α-D-半乳糖胺残基具有主要亲和力。

外觀

Contains sodium citrate

分析報告

凝集活性以 μg/mL表示,并且使用磷酸盐缓冲盐水(pH6.8)对1mg/mL溶液进行连续稀释液来测定,对于每种凝集素,所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后,对2%合适的红细胞悬浮液进行凝集所需的最低浓度。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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