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重要文件

L5142

Sigma-Aldrich

来自 普通小麦 的凝集素(小麦)

biotin conjugate, lyophilized powder

同義詞:

WGA, Wheat germ agglutinin

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
34058011
NACRES:
NA.32
暫時無法取得訂價和供貨情況

共軛

biotin conjugate

品質等級

形狀

lyophilized powder

成份

Protein, ≥80% E1%/280

標籤範圍

2-4 mol biotin per mol protein

儲存溫度

2-8°C

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相關類別

一般說明

来自Triticum vulgaris的小麦胚芽凝集素(WGA)以同型二聚体形式存在,并且具有多个N-乙酰葡糖胺(GlcNAc)结合位点。它对蛋白质变性剂具有抗性,可用于检测 O-GlcNAc蛋白。[1] WGA与N-乙醇酰神经氨酸(Neu5Ac)结合可调节促炎免疫应答。[2]

應用

来自Triticum vulgaris (小麦)的凝集素已被用于:
  • 丝虫寄生虫的子宫内阶段的样品的凝集素结合测定中[3]
  • 小鼠心肌细胞的组织学研究中[4]
  • 与Dynabeads一起形成珠子-生物素-WGA复合物® M-280链霉抗生物素蛋白磁珠,用于对来自水样中的弓形虫卵母细胞中的凝集素进行磁性分离[5]

生化/生理作用

WGA 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。

包裝

包装尺寸基于蛋白质含量。

外觀

含有柠檬酸钠

準備報告

由亲和纯化凝集素制备偶联物。

法律資訊

Dynabeads is a registered trademark of Invitrogen Dynal AS Corp

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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存取文件庫

WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins
Kubota Y, et al.
Testing, 12(7), e0180714-e0180714 (2017)
Candida Fagundes Teixeira et al.
PLoS neglected tropical diseases, 1(2), e73-e73 (2007-12-07)
Diagnosis of intestinal schistosomiasis in low endemic areas is a problem because often control measures have reduced egg burdens in feces to below the detection limits of classical coproparasitological methods. Evaluation of molecular methods is hindered by the absence of
R E Morris et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 40(5), 711-721 (1992-05-01)
We demonstrate here that the intracellular routing of biotinylated ligands was not affected by the attachment of streptavidin gold colloids so long as the electron-dense marker was added after the biotinyl ligand-receptor interaction had occurred. The binding, internalization, and intracellular
Evaluation of bi-ventricular coronary flow patterns using high-frequency ultrasound in mice with transverse aortic constriction
Wu J, et al.
Ultrasound in Medicine & Biology, 39(11), 2053-2065 (2013)
A flow cytometry based method for studying embryogenesis and immune reactivity to embryogenic stages in filarial parasites
Sahu BR, et al.
Filaria Journal, 4(1), 11-11 (2005)

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