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M3003

肌激酶 来源于兔肌肉

ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)

同義詞:

ATP: AMP 磷酸转移酶, 腺苷酸激酶

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CAS 編號:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-743-3
NACRES:
NA.54
MDL number:
EC號碼:
Specific activity:
1,500-3,000 units/mg protein (biuret)
Biological source:
rabbit muscle
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biological source

rabbit muscle

Quality Segment

form

ammonium sulfate suspension

specific activity

1,500-3,000 units/mg protein (biuret)

mol wt

21 kDa

foreign activity

3-phosphoglyceric phosphokinase ≤0.05%, lactic dehydrogenase and pyruvate kinase ≤0.01%

storage temp.

2-8°C

General description

研究领域:细胞信号传导
肌激酶,也称腺苷酸激酶或ADK,是一种磷酸转移酶。它属于核苷单磷酸激酶家族。肌激酶是一种存在于骨骼肌中的酶,可以作为磷酸转移酶刺激剂。

Application

肌激酶已用于:

  • 评估其与对羟基汞苯甲酸酯和对汞苯磺酸酯汞制剂的反应
  • 研究其肽片段的配体结合性质
  • 作为ATP生成溶液的成分用于通过AMP-荧光素酶试验定量测定3’3’-c-di-AMP

Biochem/physiol Actions

肌激酶,或称腺苷酸激酶,在细胞内能量代谢的调节以及不同细胞区室中腺嘌呤核甙酸比率的平衡中起关键作用。

肌激酶是参与腺嘌呤核甙酸代谢的关键酶。肌激酶的作用确保腺嘌呤核苷酸(ATP、ADP和AMP)在胞质溶胶中保持平衡。
肌激酶催化以下反应:ADP + ADP → ATP + AMP
使用二硫双(2-硝基苯甲酸)处理,可以使兔肌肉肌激酶失活,因为它能改变对肌激酶活性至关重要的蛋氨酸残基。

Physical form

悬浮在3.2M(NH4)2SO4溶液中,pH 6.0,含有0.001 M EDTA

Other Notes

一个酶活性单位是指在pH 7.6、37°C下,每分钟将2.0 μmole的ADP转化为ATP + AMP所需的酶量。

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本產品
P1506P9136G6751
description

ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)

description

Type II, ammonium sulfate suspension, 350-600 units/mg protein

description

Type III, lyophilized powder, 350-600 units/mg protein

description

Type I, ammonium sulfate suspension, 100-300 units/mg protein

biological source

rabbit muscle

biological source

rabbit muscle

biological source

rabbit muscle

biological source

rabbit muscle

specific activity

1,500-3,000 units/mg protein (biuret)

specific activity

350-600 units/mg protein

specific activity

350-600 units/mg protein

specific activity

100-300 units/mg protein

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

lyophilized powder

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

mol wt

21 kDa

mol wt

237 kDa

mol wt

237 kDa

mol wt

-

foreign activity

lactic dehydrogenase and pyruvate kinase ≤0.01%, 3-phosphoglyceric phosphokinase ≤0.05%

foreign activity

lactic dehydrogenase, creatine phosphokinase, and myokinase ≤0.01%, phosphoglucomutase ≤0.05%

foreign activity

lactic dehydrogenase, creatine phosphokinase, phosphoglucomutase, and myokinase ≤0.01%

foreign activity

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, Triosephosphate isomerase ≤0.02%


存儲類別/等級

10 - Combustible liquids

到底發生了什麼事?

WGK 3

閃點 (F)

Not applicable

閃點(°C)

Not applicable

PPE(個人防護裝備)

Eyeshields, Gloves, type N95 (US)



從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

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Questions

  1. M3003-1KUの容量についてお教えください。

    1 answer
    1. This product is an ammonium sulfate suspension of Myokinase. This information may be determined by values reported in the Certificate of Analysis. A survey of recent lots indicates the an average volume of 160 uL for the 1KU package size, 400 uL for the 2.5KU package size, 800 uL for the 5KU package size, and 1.6 mL for the 10KU package size. The enzyme activity may range from 1500 - 3000 units per milligram protein and the protein concentration may range from 1 - 10 mg/mL. Please see the link below to review a sample or lot-specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/m3003#product-documentation

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