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重要文件

L0770

Sigma-Aldrich

来自Pisum sativum(豌豆)的凝集素

FITC conjugate, lyophilized powder

同義詞:

Pisum sativum凝集素, PSA

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32
暫時無法取得訂價和供貨情況

共軛

FITC conjugate

品質等級

形狀

lyophilized powder

效力

<20 μg per mL agglutination activity

成份

Protein, ≥95% biuret

標籤範圍

2-4 mol FITC per mol protein (Lowry).

雜質

salt, free

儲存溫度

−20°C

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一般說明

豌豆(Pea)凝集素是一种约48kda的四聚糖蛋白。[1]它是从豌豆中分离得到。[1]豌豆凝集素对α-D-葡萄糖和α-D-甘露糖具有特异性。[1]

應用

豌豆(Pea)凝集素用于确定顶体状态[2]和精子活力[3]以及评估精子顶体反应(AR)模式[4]

生化/生理作用

PSA不是血型特异性的,但对末端α-D-甘露糖残基具有亲和力。 PSA是一种类似于伴刀豆球蛋白A的促分裂原。
来自豌豆(Pea)的凝集素对淋巴细胞(如刀豆蛋白A (Con A))具有抗肝癌作用和丝裂原活性。[5]

準備報告

由亲和纯化凝集素制备偶联物。

分析報告

凝集活性以μg/ml表示,并且是通过1mg/ml溶液的系列稀释液(pH 6.8)(在磷酸盐缓冲盐水中进行稀释)进行测定。该活性是在25℃孵育1小时后,对2%人红细胞悬浮液进行凝集所需的最低浓度。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen
Aires VA, et al.
Theriogenology, 60(2), 269-279 (2003)
The biological significance of phospholipase C beta 1 gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development.
Choi D, et al.
Journal of Assisted Reproduction and Genetics, 18(5), 305-310 (2001)
Yi Zhou et al.
Nature communications, 13(1), 2906-2906 (2022-05-26)
There is evidence to suggest that the primary tumor induces the formation of a pre-metastatic niche in distal organs by stimulating the production of pro-metastatic factors. Given the fundamental role of the pre-metastatic niche in the development of metastases, interruption
Gediendson Ribeiro de Araujo et al.
Animal reproduction science, 163, 56-62 (2015-11-04)
We evaluated the capacity of ocelot and oncilla spermatozoa to bind to the perivitelline membranes (PVMs) of hen eggs in a sperm binding assay (S-PVM). In addition, a device that improves the standardization of the assay was developed. The number
Julio A Martinez-Rodriguez et al.
Reproduction & fertility, 1(1), 11-19 (2020-11-04)
Cryopreservation compromises the capacity of sperm fertilizing due to a series of alterations in the structure and physiology of the sperm. The use of antioxidants, such as melatonin, added to freezing media, may help to reduce sperm cryoinjury. To test

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