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318337

Sigma-Aldrich

吖啶橙 盐酸盐 水合物

≥98% (HPLC)

同義詞:

3,6-双(二甲氨基)吖啶 盐酸盐

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About This Item

經驗公式(希爾表示法):
C17H19N3 · HCl · xH2O
CAS號碼:
分子量::
301.81 (anhydrous basis)
顏色索引編號:
46005
Beilstein:
3734978
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47
暫時無法取得訂價和供貨情況

品質等級

化驗

≥98% (HPLC)

形狀

powder

技術

microbe id | staining: suitable

顏色

orange

mp

284-287 °C (lit.)

溶解度

H2O: 0.1%, clear, orange to red

λmax

492 nm

&epsilon ;(消光係數)

≥14,000 at 227-233 nm in water at 0.005 g/L
≥35000 at 265-271 nm in water at 0.005 g/L

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

Cl[H].[H]O[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C

InChI

1S/C17H19N3.ClH.H2O/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;/h5-11H,1-4H3;1H;1H2

InChI 密鑰

JUAAUEXLEKAEQC-UHFFFAOYSA-N

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應用

一种严格纯化的无锌材料,适用于流式细胞术。
使用了吖啶橙盐酸盐水合物染料:
  • 染色细胞内酸性囊泡。[1]
  • 测量溶酶体膜渗透性 (LMP)。[1]
  • 或自噬检测。[2]
琼脂糖和聚丙烯酰胺凝胶中核酸的荧光染色。已用于各种细胞培养和组织模型中细胞凋亡的研究。

生化/生理作用

吖啶橙 (Acridine Orange) 是一种异染性染料,可以染色 DNA、RNA 和酸性糖胺聚糖。在低浓度时,它嵌入 DNA 并沉淀 RNA。然而,在高浓度时,它使 RNA 和 DNA 变性并沉淀。[3]吖啶橙也用于分析自噬。以 pH 依赖性方式进入酸性细胞器。在中性 pH 条件下,吖啶橙发出绿色荧光,在酸性条件下,吖啶橙在酸性细胞器中聚集,发出鲜红色荧光。[4]

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象形圖

Health hazard

訊號詞

Warning

危險聲明

危險分類

Muta. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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Questions

  1. Is product 318337, Acridine Orange hydrochloride hydrate, used for sperm chromatin structure assay (SCSA) and sperm DNA structure assay?

    1 answer
    1. Product 318337 is not function tested during quality control testing for use in either the sperm chromatin structure assay (SCSA) or the sperm DNA structure assay. The product is sold for Research Use Only. Quality Control testing includes testing for appearance, infrared spectrum, extinction coefficients at 3 different wavelengths, carbon, nitrogen, purity by HPLC, zinc, solubility, and water content. The literature shows that Acridine Orange is used for differential staining of DNA and RNA and a wide range of other uses. The website does list various uses for product 318337. The list however is not all inclusive of the uses for which the dye can be used. It is up to the customer to determine if the dye is acceptable for their intended use(s).

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