KCQS08

KiCqStart^®一步法探针RT-qPCR Readymix^™

with Low ROX for ABI and Stratagene instruments

• 同義詞: KiCqStart^® One Step Probe RT-qPCR Readymix^™
• 分類程式碼代碼: 41106300
• NACRES: NA.55

屬性

• 形狀: liquid
• 用途: sufficient for 500 reactions
• 特點: dNTPs included; hotstart
• 儲存條件: protect from light
• 技術: qPCR: suitable
• 顏色: light blue
• 輸入: purified RNA
• 相容性: ABI 7500 Fast; ABI 7500; ABI QuantStudio; ABI ViiA 7; Agilent AriaMx; Douglas Scientific IntelliQube; Qiagen Corbett Rotor-Gene Q; Strategene Mx3000P; Strategene Mx3005P; Strategene Mx4000
• 檢測方法: probe-based
• 運輸包裝: dry ice
• 儲存溫度: −20°C

描述

一般說明

KiCqStart^® One-Step Probe RT-qPCR ReadyMix^™ is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan^® 5′-hydrolysis probes on real-time PCR systems (refer to instrument compatibility section to select the appropriate reagent for your machine). First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times.

KiCqStart^® One-Step Probe RT-qPCR ReadyMix^™ contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries. The reverse transcriptase is an engineered M-MLV with reduced RNase H activity and improved activity and stability at higher temperatures. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.

KiCqStart^® One-Step Probe RT-qPCR ReadyMix^™ is highly resistant to PCR inhibitors. A key component of the ReadyMix^™ is an ultra-pure, highly processive, thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This provides an extremely stringent automatic hot-start that minimizes the potential for primer-dimer and other non-specific PCR artifacts. The light blue color of the inert dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. The dye does not interfere with qPCR performance or affect the stability of the product.

應用

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart^® One-Step Probe RT-qPCR ReadyMix^™ does not contain an internal reference dye. Compatible instruments include:

• Applied Biosystems^® 7500
• Applied Biosystems 7500 Fast
• Applied Biosystems ViiA 7
• Applied Biosystems QuantStudio
• Agilent^® AriaMx
• Douglas Scientific IntelliQube
• QIAGEN^® Rotor-Gene^® Q
• Stratagene Mx3000P^®
• Stratagene Mx3005P^®
• Stratagene Mx4000^™

特點和優勢

• 仅需33分钟即可获得检测结果
• 高效和高灵敏度的实时PCR结果
• 极少/无需优化

成分

2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers

packaging:
500 reactions* = 5 X 1 mL tubes
*number of reactions based on a 20uL volume

法律資訊

Agilent is a registered trademark of Agilent Technologies, Inc.

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

KiCqStart is a registered trademark of QIAGEN Beverly Inc.

Mx3000P is a registered trademark of Agilent Technologies, Inc.

Mx3005P is a registered trademark of Agilent Technologies, Inc.

Mx4000 is a trademark of Agilent Technologies, Inc.

Qiagen is a registered trademark of Qiagen Group

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Rotor-Gene is a registered trademark of Qiagen GmbH

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable