This product is tested for the expansion of TF-1 cells. The activity is reported as the ED50 in nanograms per milliliter. The activity in International Units has not been established. The ED50 value is reported on the lot-specific Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/h5166#product-documentation
推薦產品
生物源
human
品質等級
重組細胞
expressed in HEK 293 cells
化驗
≥95% (SDS-PAGE)
形狀
lyophilized powder
效力
≤5.0 ng/mL ED50
品質
endotoxin tested
分子量
dimer 36 kDa (glycosylated)
包裝
pkg of 10 μg
儲存條件
avoid repeated freeze/thaw cycles
技術
cell culture | mammalian: suitable
雜質
≤1 EU/μg
UniProt登錄號
儲存溫度
−20°C
基因資訊
human ... EPO(2056)
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相關類別
一般說明
EPO has been cloned from various species including human, murine, canine, and others. The mature proteins from the various species are highly conserved and exhibit greater than 80% amino acid sequence identity. EPO contains three N-linked glycosylation sites. The glycosylation of erythropoietin is required for the biological activities of erythropoietin in vivo.
生化／生理作用
Erythropoietin is a glycoprotein that is the principal regulator of red blood cell growth and differentiation.
Erythropoietin (EPO), produced primarily by the kidney, is the primary regulatory factor of erythropoiesis. It promotes the proliferation, differentiation, and survival of the erythroid progenitors. Erythropoietin stimulates erythropoiesis by inducing growth and differentiation of burst forming units and colony forming units into mature red blood cells. EPO produced by kidney cells is increased in response to hypoxia or anemia. The biological effects of erythropoietin are mediated by the erythropoietin receptor, which binds EPO with high affinity and is a potent EPO antagonist.
準備報告
Human EPO is expressed as a glycosylated 36 kDa monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
分析報告
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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文章
Read article on hematopoietic cytokines and hematopoiesis
列出主要作用似乎是刺激或調節造血細胞的細胞激素，包括 GM-CSF、G-CSF、M-CSF、白細胞介素、EPO 和 TPO。
條款
生成人類誘導多能幹細胞 (iPSC) 的幹細胞重編方案，包括以病毒和非病毒 RNA 為基礎的方法。
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
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Hi, we recently ordered this product, and I wonder how much international unit for this product of 10ug?
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