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來源證明/COA

GE80-6484-51

2-D Clean-Up Kit

Name: 2-D Clean-Up Kit
Brand: SIGMA

Cytiva 80-6484-51

同義詞:

2D Clean-Up Kit

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About This Item

分類程式碼代碼:

41105323

NACRES:

NA.25

暫時無法取得訂價和供貨情況

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特點

wetted part: no

製造商／商標名

Cytiva 80-6484-51

包裝

pkg of 50 samples

一般說明

2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.

Treatment of the sample with 2-D Clean-Up Kit can improve the quality of 2-D electrophoresis results, reducing streaking, background staining, and other artifacts due to interfering contaminants. The kit can make 2-D analysis possible for samples that are otherwise too dirty or too dilute.

The 2-D Clean-Up Kit procedure uses precipitant and co-precipitant in combination to quantitatively precipitate proteins. Precipitated proteins are pelleted by centrifugation and the precipitate is further washed to remove nonprotein contaminants. After a second centrifugation, the resultant pellet is resuspended into denaturing sample solution for first-dimension IEF.

The procedure can be completed in 90 min and does not result in spot gain or loss. The kit contains sufficient reagents to process 50 samples of up to 100 μ each. The procedure can be scaled up for larger volumes or more dilute samples.

2-D Clean-Up Kit increases labeling efficiency and protein abundance when using 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE).

應用

Automated identification and quantitation of proteins and peptides using LC-MS/MS.

特點和優勢

Improves the quality of 2-D electrophoresis results by removing interfering contaminants
Quantitative precipitation separates proteins from detergents, salts, lipids, phenolics and nucleic acids
Delivers quantitative yield in about 90 min.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

The wash buffer should be placed at -20 °C at least 1 h before use and may be s-red in a -20 °C freezer