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重要文件

DFF100

Sigma-Aldrich

DEAE - 琼脂糖凝胶

Fast Flow

同義詞:

二乙氨乙基 - 琼脂糖凝胶

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
47101511
NACRES:
NA.56
暫時無法取得訂價和供貨情況

品質等級

形狀

suspension

技術

affinity chromatography: suitable

基質

6% cross-linked agarose

珠尺寸

45-165 μm (wet)

孔徑

~4,000,000 Da exclusion limit

pH值

2—12

容量

110-160 μeq/mL binding capacity(gel volume)

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一般說明

DFF100-500Ml′ 的更新产品编号为 GE17-0709-01

應用

DEAE - 琼脂糖凝胶用于亲和层析、蛋白质层析和离子交换层析。 DEAE - 琼脂糖凝胶已被用于研究人类疾病的发病机制,并开发了一种新的检测方法,用于检测艰难梭菌致病菌株的毒素。
DEAE-琼脂糖凝胶用于:
  • 阴离子交换色谱
  • 筛选聚异戊二烯磷酸磷酸酶活性[1]
  • 纯化同工抑制剂[2]
  • 纯化人类免疫缺陷病毒(HIV)
  • 糖蛋白包膜(gp140 env)[3]

生化/生理作用

二乙氨乙基-琼脂糖凝胶(DEAE-琼脂糖凝胶)是强阴离子交换柱,其中DEAE与琼脂糖共价结合。[4]

法律資訊

Sepharose is a trademark of Cytiva

取代透過

象形圖

Flame

訊號詞

Warning

危險聲明

危險分類

Flam. Liq. 3

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

100.4 - 109.4 °F

閃點(°C)

38 - 43 °C

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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分析證明 (COA)

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存取文件庫

Jessie Fernandez et al.
mBio, 12(1) (2021-02-11)
Rice blast disease caused by Magnaporthe oryzae is a devastating disease of cultivated rice worldwide. Infections by this fungus lead to a significant reduction in rice yields and threats to food security. To gain better insight into growth and cell
G L Yewey et al.
Biochemical and biophysical research communications, 148(3), 1520-1526 (1987-11-13)
Bovine heart cytochrome c oxidase, depleted of polypeptide subunits by alkaline detergent treatment, was characterized with respect to metal content, optical spectral properties, and oxidase activity. Treatment with 1.0% Triton X-100 at pH 9.5 followed by anion-exchange chromatography caused removal
T M Schmidt et al.
Applied and environmental microbiology, 55(10), 2607-2612 (1989-10-01)
Luminescence of batch cultures of Xenorhabdus luminescens was maximal when cultures approached stationary phase; the onset of in vivo luminescence coincided with a burst of synthesis of bacterial luciferase, the enzyme responsible for luminescence. Expression of luciferase was aldehyde limited
Advances in Potato Chemistry and Technology (2016)
Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems
Rosenberg Y, et al.
Testing, 8(3), e58724-e58724 (2013)

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