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GE17-5110-01

HiTrap® IGM purification

Cytiva 17-5110-01, pack of 5 × 1 mL

同義詞:

IGM Purification Column

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56
暫時無法取得訂價和供貨情況

ligand

2-mercaptopyridine

包裝

pack of 5 × 1 mL

製造商／商標名

Cytiva 17-5110-01

儲存條件

(20% Ethanol)

參數

<4 mL/min flow rate
42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

16 mm

基質

highly cross-linked 6% agarose

樣品平均尺寸

34 μm

cleaning

2-13

工作範圍

3-11

儲存溫度

2-8°C

一般說明

HiTrap® IgM Purification HP is a 1 mL column prepacked with Sepharose™ High Performancefor fast and efficient purification of monoclonal IgM.

應用

HiTrap® IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

特點和優勢

  • Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
  • Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance.
  • Recovery of IgM is high with excellent activity retention.
  • The top and bottom frits are manufactured from porous polyethylene
  • Columns are delivered with a stopper on the inlet and a snap-off end on the outle

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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分析證明 (COA)

Lot/Batch Number

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文章

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

抗體親和層析的脫鹽和緩衝液交換

在實驗室規模下進行脫鹽是一種行之有效、簡單且快速的方法，可快速去除低分子量雜質，同時只需一個步驟即可將樣品轉移到所需的緩衝液中。

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Performing a Separation with HiTrap® IgM Purifcation HP

How to perform a separation with the HiTrap® IgM Purifcation HP from Cytiva which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from Cytiva.

親和層析的緩衝液交換和脫鹽

如何使用 Sephadex G-25、HiTrap Desalting columns 或 ÄKTAprime plus 進行緩衝液交換和脫鹽。

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

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相關內容

Performing a Separation with HiTrap® IgM Purifcation HP

How to perform a separation with the HiTrap IgM Purifcation HP from GE Healthcare which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

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