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重要文件

I8260

Sigma-Aldrich

IgM 来源于人类血清

reagent grade, ~95% (HPLC), buffered aqueous solution

同義詞:

人IgM

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46
暫時無法取得訂價和供貨情況

共軛

unconjugated

等級

reagent grade

化驗

~95% (HPLC)

形狀

buffered aqueous solution

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

一般說明

人体有五种免疫球蛋白。其中,免疫球蛋白M (IgM)是一种高分子量蛋白质,包括五或六个亚单位。IgM单体由两条重链和两条轻链通过二硫键连接而成。人血清中IgM单体浓度低。它具有大约12%的高碳水化合物含量。这是新生儿产生的第一种免疫球蛋白。[1]
IgM抗体在血清中以五聚体的形式存在,为响应抗原而产生。[2]
通过沉淀和凝胶过滤技术从正常人血清中纯化人血清中的IgM。通过HPLC流程测定免疫球蛋白纯度至少为95%。

應用

免疫原IgM可在各种免疫测定中用作参考抗原、标准、封闭剂或外壳蛋白,包括ELISA、斑点免疫结合、免疫印迹、免疫扩散和免疫电泳。其他应用包括制备免疫原和固相免疫吸附剂的起始物料。人血清中的IgM作为ELISA (100 μg/ml)[3]和斑点印迹法标准。[4]在IgMIgM结合试验中使用200 μg/ml至1 mg/ml的抗体浓度范围。[5][6]
人血清中的IgM已
  • 用于ELISA抑制试验[7]
  • 用于免疫印迹[7]
  • 用作分子量(MW)标准[8]
  • 用于IgM结合试验[9]
  • 用于确认免疫球蛋白耗竭[10]

生化/生理作用

免疫球蛋白M (IgM)以其单体形式作为未受刺激的淋巴细胞表面上的B细胞抗原受体的抗原特异性部分。聚合IgM分子也是经典补体级联的重要激活剂。IgM在凝集和细胞溶解反应中是必不可少的。[1]

外觀

在0.05 M三羟甲基氨基甲烷盐酸盐、0.2 M氯化钠溶液中,pH 8.0,含有15 mM叠氮化钠。

儲存和穩定性

储存在2-8 °C条件下。如果长期储存时出现轻微混浊,使用前通过离心澄清溶液。

免責聲明

除非在我们的产品目录或产品随附的其他公司文档中另有说明,我们的产品仅供研究使用并不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体体内治疗用途、任何类型的食用或应用于人或动物。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Localization of the domains of the Haemophilus ducreyi trimeric autotransporter DsrA involved in serum resistance and binding to the extracellular matrix proteins fibronectin and vitronectin.
Leduc I, et al.
Infection and Immunity, 77(2), 657-666 (2009)
Selection of mimotopes of the cell surface adhesion molecule Mel-CAM from a random pVIII-28aa phage peptide library.
Hafner C, et al.
The Journal of Investigative Dermatology, 119(4), 865-869 (2002)
Apolline Salama et al.
Transplantation, 101(10), 2501-2507 (2017-02-16)
Polyclonal antihuman thymocyte rabbit IgGs (antithymocyte globulin [ATG]) are popular immunosuppressive drugs used to prevent or treat organ or bone-marrow allograft rejection, graft versus host disease, and autoimmune diseases. However, animal-derived glycoproteins are also strongly immunogenic and rabbit ATG induces
L Rydberg et al.
Xenotransplantation, 5(2), 105-110 (1998-12-16)
A quantitative ELISA technique for determination of human anti-pig xenoantibody number in serum samples has been established using pig lymphocytes and pig/rabbit erythrocytes as target cells and a pool of serum from human blood group AB donors. The number of
Isabelle Leduc et al.
Infection and immunity, 77(2), 657-666 (2008-11-19)
Resisting the bactericidal activity of naturally occurring antibodies and complement of normal human serum is an important element in the evasion of innate immunity by bacteria. In the gram-negative mucosal pathogen Haemophilus ducreyi, serum resistance is mediated primarily by the

Questions

  1. What is the molecular weight of the IgM antibody from human serum with the product code I8260?

    1 answer
    1. The IgM antibody with product code I8260 is not tested for molecular weight, as it consists of two heavy and two light chains. For the unmodified human IgM subunit chains, one publication reports the following molecular weights: m chain = 75 kDa, k chain = 22 kDa. Refer to the publication "Habeeb, A.F.S.A., et al., Studies of Maleylated Chains of Human IgM. J. Immunol., 105, 846-855 (1970)" for more information. Additionally, the monomer of the IgA product with code I4036 is expected to have a molecular weight of 160 kDa.

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