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G9382

Sigma-Aldrich

明胶 来源于牛皮

gel strength ~225 g Bloom, Type B

同義詞:

牛明胶

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.26
暫時無法取得訂價和供貨情況

生物源

bovine skin

品質等級

種類

Type B

化驗

≥95% protein basis (biuret)

形狀

powder

技術

ELISA: suitable
cell culture | mammalian: suitable
immunocytochemistry: suitable
western blot: suitable

溶解度

H2O: soluble 50 mg/mL

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成分

明胶存在于胶原蛋白中,是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。

注意

干燥的明胶,当在室温下储存于密闭容器中时,将可保持多年不变。 当在空气存在时在100℃下加热时,它会溶胀变软,并分解成碳质物质并伴有吡啶碱和氨的释放。

準備報告

本品由牛皮制成。明胶可溶于热水而非冷水。 它几乎不溶于大多数有机溶剂,如醇、氯仿、二硫化碳、四氯化碳、乙醚、苯、丙酮和油。 由Bloom凝胶计所测定的Bloom指数,可表示由已知浓度溶液而形成的凝胶的强度。 Bloom指数与平均分子量成正比。 猪皮明胶的Bloom指数变化于90至300 g之间。该产品具有225的凝胶强度。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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K A van der Lee et al.
Journal of lipid research, 41(1), 41-47 (2000-01-11)
Long-chain fatty acids are the most important substrates for the heart. In addition, they have been shown to affect signalling pathways and gene expression. To explore the effects of long-chain fatty acids on cardiac gene expression, neonatal rat ventricular myocytes
Ochuko L Erukainure et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 83, 1478-1484 (2016-10-25)
The immunomodulatory potentials of the crude methanolic extract and fractions [n-hexane (Hex), n-dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol (BuOH)] of Clerodendrum volubile flowers were investigated on whole blood phagocytic oxidative burst using luminol-amplified chemiluminescence technique. They were also investigated
T H Kim et al.
Hepatology (Baltimore, Md.), 31(1), 75-82 (1999-12-29)
Partial hepatectomy triggers a variety of biological phenomena, which culminate in regeneration of the liver mass. Hepatocyte proliferation is a major feature of the regenerating liver after partial hepatectomy. Previous studies in our laboratory suggested that hepatic matrix remodeling might
Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)
Silva-Pinhati A C O, et al.
Neotropical Entomology, 34, 1-5 (2005)
Comparison of γ-radiation and electron beam irradiation effects on gelatin.
Vieira FF, et al.
Radiation Physics and Chemistry, 63, 331-332 (2002)

Questions

1–10 of 11 Questions  
  1. What is the bulk density of this product

    1 answer
    1. The bulk density of this product is not determined.

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  2. What is the molecular weight of this Gelatin?

    What is the molecular weight of this Gelatin?

    1 answer
    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

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  3. How to sterilize 2% solution?

    1 answer
    1. This item can be sterilized by autoclaving at 121°C, 15 psi for 30 minutes.

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  4. Which is the molecular weight?

    1 answer
    1. The Bloom number or gel strength of this product is ~225 g Bloom. The average molecular mass of 175-225 g Bloom (Medium Bloom) products is 40,000-50,000 Da. The average molecular mass of 225-325 g Bloom (High Bloom) products is 50,000-100,000 Da. Therefore, this product is expected to have a molecular weight of approximately 50 kDa.

      For more information please view the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/614/g6650-et-al-pis-ms.pdf

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  5. Is this made from skin from a cow which was killed for the food chain?

    1 answer
    1. All information that is shared regarding source can be found on a lot-specific Certificate of Origin (CofO, COO) for this product.

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  6. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  7. What is Product G9382, Gelatin from bovine skin, soluble in?

    1 answer
    1. Gelatin is soluble in glycerol and acetic acid, and more soluble in hot water than in cold water.  We test the solubility of gelatin in water at 50 mg/mL (with heat).

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  8. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  9. What is a Bloom number, and how does it correlate with the molecular weight?

    1 answer
    1. The Bloom number is indicative of gel strength. The Bloom number is the amount of pressure, in grams, required to break the surface of a 6.67% gel. The general rule of thumb in regards to an average molecular weight: High Bloom (225-325) = 50,000-100,000; Medium Bloom (175-225) = 40,000-50,000; Low Bloom (50-125) = 20,000-25,000. Product G9382 is a high Bloom preparation of approximately 225 Bloom.

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  10. What is the difference between type A and type B gelatin?

    1 answer
    1. Type A gelatin is derived from acid-cured tissue whereas Type B gelatin is derived from lime-cured tissue. Type A gelatin has about 78-80 millimoles of free carboxyl groups per 100 g of protein and a pI of 7.9-9.0. Type B has 100-115 millimoles of free carboxyl groups per 100 g of protein and a pI of 4.7-5.2.

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1–10 of 11 Questions  

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